Faix R G, Finkel D J, Andersen R D, Hostetter M K
Department of Pediatrics, University of Michigan, Ann Arbor, USA.
Pediatr Infect Dis J. 1995 Dec;14(12):1063-8. doi: 10.1097/00006454-199512000-00007.
Five very low birth weight infants developed systemic Candida albicans infection during a 6-week period in an urban neonatal intensive care unit. In an effort to assess whether a common source outbreak was present, the genomic DNA of the clinical isolates was compared by EcoRI and XbaI restriction fragment analysis and Southern blot hybridization with 27A, a species-specific, transposon-like probe. Although the restriction fragment analysis suggested strong similarities among the isolates, the hybridization patterns demonstrated that all strains were genotypically distinct. The parallel use of at least two restriction enzymes was important for differentiating the isolates. Rapid genotypic analysis of clinical isolates from a cluster of C. albicans infections permits determination of whether a common source is probable, facilitates epidemiologic decisions and may reduce infection control measures and attendant costs.
在一个城市新生儿重症监护病房的6周时间里，5名极低出生体重儿发生了白色念珠菌全身性感染。为了评估是否存在共同来源的暴发，通过EcoRI和XbaI限制性片段分析以及用物种特异性的转座子样探针27A进行Southern印迹杂交，对临床分离株的基因组DNA进行了比较。尽管限制性片段分析表明分离株之间有很强的相似性，但杂交模式显示所有菌株在基因型上都是不同的。至少使用两种限制性酶进行平行分析对于区分分离株很重要。对一组白色念珠菌感染的临床分离株进行快速基因型分析，有助于确定是否可能存在共同来源，便于做出流行病学决策，并可能减少感染控制措施及相关成本。
