Doi M, Chibana H, Nakagawa Y, Tanaka K
Laboratory of Medical Mycology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Aichi, Japan.
Microbiol Immunol. 1998;42(3):227-30. doi: 10.1111/j.1348-0421.1998.tb02275.x.
A primer pair, PB and BSH, which amplified alts, a portion of Candida albicans-specific repetitive sequence, RPS, gave stable and reproducible fingerprint patterns of the strains by polymerase chain reaction (PCR). We applied this method to clinical isolates of C. albicans for strain discrimination. Using PCR fingerprint patterns, we could analyze the relatedness of C. albicans strains including those isolated from children with leukemia and their bedside parents. The results indicated that PCR analysis targeting an alt region gives rise to the same conclusion as the previous study obtained by SmaI RFLP analysis.
一对引物PB和BSH可扩增出白色念珠菌特异性重复序列RPS的一部分alts，通过聚合酶链反应（PCR）可得到该菌株稳定且可重复的指纹图谱。我们将此方法应用于白色念珠菌临床分离株的菌株鉴别。利用PCR指纹图谱，我们能够分析白色念珠菌菌株之间的相关性，包括从白血病患儿及其床边父母处分离得到的菌株。结果表明，针对alts区域的PCR分析得出的结论与之前通过SmaI限制性片段长度多态性（RFLP）分析得到的结论相同。
