Ye Ya-Jing, Wang Peng-Yan, Li Ya-Peng, Yin Da-Chuan
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, 710072, China.
J Mater Sci Mater Med. 2015 Feb;26(2):81. doi: 10.1007/s10856-015-5419-1. Epub 2015 Jan 30.
Eutectic melting bonding (EMB) method is a useful technique for fabricating bioactive coatings with relatively high crystallinity and bonding strength with substrate on titanium substrates. Using the EMB method, hydroxyapatite/Ti2Ni coatings were prepared on the surface of Ti-6Al-4V at a relatively low temperature (1,050 °C) in a vacuum furnace. The coatings were then characterized in terms of phase components, microstructure, bonding strength and cytotoxicity. The results showed that the coatings were mainly composed of HAp and Ti2Ni, and the thickness of the coatings was approximately 300 μm. X-ray diffraction analysis showed that the coatings exhibited relatively high crystallinity. The tensile bonding strength between the coatings and the substrates was 69.68±5.15 MPa. The coatings had a porous and rough surface which is suitable for cell attachment and filopodia growth. The cell culture study showed that the number of MG-63 cells increased, and the cell morphology changed with the incubation time. This study showed that the EMB method can be utilized as a potentially powerful method to obtain high quality hydroxyapatite coatings with desired mechanical and biocompatibility properties on Ti-alloy substrates.
共晶熔融结合（EMB）法是一种在钛基体上制备具有相对较高结晶度且与基体结合强度高的生物活性涂层的有用技术。采用EMB法，在真空炉中于相对较低温度（1050℃）下在Ti-6Al-4V表面制备了羟基磷灰石/Ti2Ni涂层。然后对涂层的相组成、微观结构、结合强度和细胞毒性进行了表征。结果表明，涂层主要由HAp和Ti2Ni组成，涂层厚度约为300μm。X射线衍射分析表明涂层具有较高的结晶度。涂层与基体之间的拉伸结合强度为69.68±5.15MPa。涂层表面多孔且粗糙，适合细胞附着和丝状伪足生长。细胞培养研究表明，MG-63细胞数量增加，且细胞形态随培养时间而变化。本研究表明，EMB法可作为一种潜在的强大方法，在钛合金基体上获得具有所需机械性能和生物相容性的高质量羟基磷灰石涂层。
