Lee Gi-Ja, Park Hun-Kuk
Department of Biomedical Engineering and Healthcare Industry Research Institute, School of Medicine, Kyung Hee University, 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 130-701, South Korea.
Methods Mol Biol. 2015;1265:397-406. doi: 10.1007/978-1-4939-2288-8_29.
Atomic force microscopy (AFM) has become an important medical and biological tool for the noninvasive imaging of cells and biomaterials in medical, biological, and biophysical research. The major advantages of AFM over conventional optical and electron microscopes for bio-imaging include the facts that no special coating is required and that imaging can be done in all environments-air, vacuum, or aqueous conditions. In addition, it can also precisely determine pico-nano Newton force interactions between the probe tip and the sample surface from force-distance curve measurements.It is widely known that mitochondrial swelling is one of the most important indicators of the opening of the mitochondrial permeability transition (MPT) pore. As mitochondrial swelling is an ultrastructural change, quantitative analysis of this change requires high-resolution microscopic methods such as AFM. Here, we describe the use of AFM-based shape analysis for the characterization of nanostructural changes in heart mitochondria resulting from myocardial ischemia-reperfusion injury.
原子力显微镜（AFM）已成为医学、生物学和生物物理研究中用于细胞和生物材料无创成像的重要医学和生物学工具。与用于生物成像的传统光学显微镜和电子显微镜相比，AFM的主要优势包括无需特殊涂层以及可在所有环境（空气、真空或水性条件）下进行成像。此外，它还能通过力-距离曲线测量精确确定探针尖端与样品表面之间的皮牛至纳牛力相互作用。众所周知，线粒体肿胀是线粒体通透性转换（MPT）孔开放的最重要指标之一。由于线粒体肿胀是一种超微结构变化，对这种变化进行定量分析需要高分辨率显微镜方法，如AFM。在此，我们描述了基于AFM的形状分析在表征心肌缺血再灌注损伤导致的心脏线粒体纳米结构变化中的应用。
