Fadhlaoui Khaled, Ben Hania Wajdi, Postec Anne, Fauque Guy, Hamdi Moktar, Ollivier Bernard, Fardeau Marie-Laure
Laboratoire d'Ecologie et de Technologie Microbienne, Institut National des Sciences Appliquées et de Technologie, Centre Urbain Nord, BP 676, 1080 Tunis, Tunisia.
Aix Marseille Université, IRD, Université de Toulon, CNRS, Mediterranean Institute of Oceanography (MIO), UM 110, 13288 Marseille, cedex 09, France.
Int J Syst Evol Microbiol. 2015 Apr;65(Pt 4):1256-1261. doi: 10.1099/ijs.0.000088. Epub 2015 Jan 29.
A novel anaerobic, mesophilic, slightly halophilic sulfate-reducing bacterium, designated strain Khaled BD4(T), was isolated from waters of a Tunisian thermal spring. Cells were vibrio-shaped or sigmoids (5-7×1-1.5 µm) and occurred singly or in pairs. Strain Khaled BD4(T) was Gram-stain-negative, motile and non-sporulated. It grew at 25-45 °C (optimum 37 °C), at pH 5.5-8.3 (optimum pH 7.0) and with 0.5-8% NaCl (optimum 3%). It required vitamins or yeast extract for growth. Sulfate, thiosulfate, sulfite and elemental sulfur served as terminal electron acceptors, but not fumarate, nitrate or nitrite. Strain Khaled BD4(T) utilized H2 in the presence of 2 mM acetate (carbon source), but also lactate, formate, pyruvate and fumarate in the presence of sulfate. Lactate was incompletely oxidized to acetate. Amongst substrates used, only pyruvate was fermented. Desulfoviridin and c-type cytochrome were present. The G+C content of the DNA was 54.6 mol%. The main fatty acids were anteiso -C(15 : 0), iso-C(18 : 0), iso-C(17 : 0) and iso-C(14 : 0). Phylogenetic analysis of the 16S rRNA gene sequence indicated that strain Khaled BD4(T) had Desulfovibrio giganteus DSM 4123(T) (96.7% similarity) as its closest phylogenetic relative. On the basis of 16S rRNA gene sequence comparisons together with genetic and physiological characteristics, strain Khaled BD4(T) is assigned to a novel bacterial species, for which the name Desulfovibrio biadhensis sp. nov. is proposed. The type strain is Khaled BD4(T) ( = DSM 28904(T) = JCM 30146(T)).
从突尼斯一处温泉水中分离出一株新型厌氧、嗜温、轻度嗜盐的硫酸盐还原菌，命名为哈立德BD4(T)菌株。细胞呈弧状或S形（5-7×1-1.5 µm），单个或成对出现。哈立德BD4(T)菌株革兰氏染色阴性，具运动性，不产芽孢。其生长温度为25-45 °C（最适温度37 °C），pH值为5.5-8.3（最适pH值7.0），NaCl浓度为0.5-8%（最适浓度3%）。生长需要维生素或酵母提取物。硫酸盐、硫代硫酸盐、亚硫酸盐和元素硫可作为末端电子受体，但富马酸盐、硝酸盐或亚硝酸盐则不行。哈立德BD4(T)菌株在有2 mM乙酸盐（碳源）存在时利用氢气，在有硫酸盐存在时也利用乳酸盐、甲酸盐、丙酮酸盐和富马酸盐。乳酸盐不完全氧化为乙酸盐。在所使用的底物中，只有丙酮酸盐可被发酵。存在脱硫绿素和c型细胞色素。DNA的G+C含量为54.6 mol%。主要脂肪酸为anteiso-C(15 : 0)、iso-C(18 : 0)、iso-C(17 : 0)和iso-C(14 : 0)。对16S rRNA基因序列的系统发育分析表明，哈立德BD4(T)菌株与巨大脱硫弧菌DSM 4123(T)（相似度96.7%）是最近的系统发育亲缘关系。基于16S rRNA基因序列比较以及遗传和生理特征，哈立德BD4(T)菌株被归为一个新的细菌物种，为此提出名为双黏脱硫弧菌（Desulfovibrio biadhensis sp. nov.）的名称。模式菌株为哈立德BD4(T)（=DSM 28904(T)=JCM 30146(T)）。
