Ma Xianyong, Zheng Chuntian, Hu Youjun, Wang Li, Yang Xuefen, Jiang Zongyong
Institute of Animal Science; Guangdong Academy of Agricultural Sciences, Guangzhou, China; State Key Laboratory of Livestock and Poultry Breeding, Guangzhou, China; Key Laboratory of Animal Nutrition and Feed Science in South China, Ministry of Agriculture, Guangzhou, China; Guangdong Public Laboratory of Animal Breeding and Nutrition, Guangzhou, China; Guangdong Key Laboratory of Animal Breeding and Nutrition, Guangzhou, China.
PLoS One. 2015 Jan 30;10(1):e0117294. doi: 10.1371/journal.pone.0117294. eCollection 2015.
Forty-eight Duroc x Landrace x Large White gilts were used to determine the relationship between proteome changes of longissimus muscle and intramuscular fat (IMF) content in arginine-supplemented pigs. Beginning at 60 kg BW, pigs were fed a corn- and soybean meal-based diet supplemented or not with 1% L-arginine until they reached a BW of 100 kg. Supplementation with 1% L-arginine did not affect the growth performance or carcass traits, while it increased IMF content by 32% (P < 0.01), it also decreased the drip loss at 48 h post-mortem and the b* meat color value at 24 h post-mortem; supplementation with 1% dietary L-arginine did not change the proportion of SFA and MUFA in muscle lipids. The proteome changes in longissimus muscle between the control and supplemented pigs showed that L-arginine significantly influenced the abundance of proteins related to energy metabolism, fiber type and structure. The increase in IMF content was positively correlated with the increased abundance of slow twitch troponin I (TNNI1) protein and negatively correlated with myosin heavy chain IIb (MyHC IIb) protein content. It is suggested that the proteome changes in longissimus muscle contributed to the greater IMF content in L-arginine supplemented pigs.
选用48头杜洛克×长白×大白母猪来确定补充精氨酸的猪的背最长肌蛋白质组变化与肌内脂肪（IMF）含量之间的关系。从体重60 kg开始，给猪饲喂以玉米和豆粕为基础的日粮，添加或不添加1%的L-精氨酸，直至体重达到100 kg。补充1%的L-精氨酸不影响生长性能和胴体性状，但IMF含量增加了32%（P<0.01），同时也降低了宰后48 h的滴水损失和宰后24 h的肉色b*值；日粮中补充1%的L-精氨酸不会改变肌肉脂质中饱和脂肪酸（SFA）和单不饱和脂肪酸（MUFA）的比例。对照猪和补充精氨酸猪的背最长肌蛋白质组变化表明，L-精氨酸显著影响与能量代谢、纤维类型和结构相关的蛋白质丰度。IMF含量的增加与慢肌肌钙蛋白I（TNNI1）蛋白丰度的增加呈正相关，与肌球蛋白重链IIb（MyHC IIb）蛋白含量呈负相关。研究表明，背最长肌的蛋白质组变化导致了补充精氨酸的猪具有更高的IMF含量。
