Bozynski Chantelle C, Kuroki Keiichi, Stannard James P, Smith Patrick A, Stoker Aaron M, Cook Cristi R, Cook James L
Comparative Orthopaedic Laboratory, University of Missouri, Columbia, Missouri.
Department of Orthopaedic Surgery, University of Missouri Hospital, Columbia, Missouri.
J Knee Surg. 2015 Oct;28(5):404-10. doi: 10.1055/s-0035-1544975. Epub 2015 Jan 30.
A major hurdle in investigating important clinical questions in knee ligament treatment is a lack of valid translational animal models. This study characterizes the effects of partial transection versus synovial debridement of the anterior (cranial) cruciate ligament (ACL) in dogs. A total of 27 adult purpose-bred research hounds underwent surgery and were assessed over the following 8 weeks. Dogs were randomized into the following three ACL status groups: sham control (n = 9), intact ACL with synovial debridement (exposed ACL) (n = 9), and partial transection of the ACL (partial tear ACL) (n = 9). Dogs in the exposed ACL group and partial tear ACL group had significantly (p < 0.05) more severe lameness, pain, effusion, reduced function, and reduced comfortable range of motion compared with controls, with the partial tear ACL group being most severely affected. More severe ACL and whole-joint pathology, and radiographic scores for osteoarthritis were present in the partial tear ACL group compared with exposed and/or sham control group. On the basis of these findings, biologic components of ACL injury (exposed ACL) played a role in whole-joint inflammation, but the clinical and pathological effects were more severe when both biologic and biomechanical components were present (i.e., partial tear ACL). These novel canine models were successfully developed to evaluate partial transection versus synovial debridement of the ACL and these models will be used to evaluate treatment options for acute management of ACL injuries.
在研究膝关节韧带治疗中的重要临床问题时，一个主要障碍是缺乏有效的转化动物模型。本研究描述了犬前交叉韧带（ACL）部分横断与滑膜清创的效果。共有27只成年专用繁殖研究猎犬接受手术，并在接下来的8周内进行评估。犬被随机分为以下三个ACL状态组：假手术对照组（n = 9）、完整ACL伴滑膜清创（暴露ACL）组（n = 9）和ACL部分横断组（部分撕裂ACL）（n = 9）。与对照组相比，暴露ACL组和部分撕裂ACL组的跛行、疼痛、积液、功能降低和舒适活动范围减小更为显著（p < 0.05），部分撕裂ACL组受影响最为严重。与暴露和/或假手术对照组相比，部分撕裂ACL组存在更严重的ACL和全关节病理改变以及骨关节炎的影像学评分。基于这些发现，ACL损伤的生物学成分（暴露ACL）在全关节炎症中起作用，但当生物学和生物力学成分都存在时（即部分撕裂ACL），临床和病理影响更为严重。这些新型犬模型已成功开发，用于评估ACL的部分横断与滑膜清创，这些模型将用于评估ACL损伤急性处理的治疗选择。
