Dai Qing-gang, Fang Bing, Zhang Peng, Yang Xiao, Wang Jie, Ouyang Ning-juan, Wu Yu-qiong, Jiang Ling-yong
Department of Oral and Craniomaxillofacial Science, Ninth People's Hospital, College of Stomatology, Shanghai Jiao Tong University School of Medicine; Shanghai Key Laboratory of Stomatology. Shanghai 200011, China.E-mail:
Shanghai Kou Qiang Yi Xue. 2014 Dec;23(6):641-5.
To study the changes of microarchitecture of alveolar bone due to different duration of ovariectomy in rats.
Twenty-four virgin Sprague-Dawley rats were randomly assigned to ovariectomy group (OVX) or sham-ovariectomy group (sham). OVX rats were subjected to bilateral ovariectomy and sham-ovariectomy was conducted in sham rats. Six rats of each group were sacrificed respectively 3 months and 6 months after surgery. The right semi-maxilla of all rats were scanned by Micro-CT, and the inter-radicular alveolar bone of the maxillary first molar was analyzed. Statistical analysis was carried out with SPSS 16.0 software package.
Two-dimensional and three-dimensional reconstructed images of the alveolar bone showed porotic changes in rats both 3 months and 6 months after ovariectomy, including thinner, looser trabeculae and expanded bone marrow. When compared with corresponding sham rats, BMD, BV/TV and Tb.Th of alveolar bone significantly decreased in OVX rats both 3 months and 6 months after ovariectomy (P<0.05). Tb.N and Tb.Sp significantly increased in both OVX groups (P<0.05). When compared with 3 months after ovariectomy, the rats 6 months after ovaroectomy shared deceased BMD, BV/TV and Tb.Th (P<0.05) and increased Tb.N (P<0.05).
Bone loss and deterioration of trabeculae of alveolar bone aggravates with the extended duration of ovariectomy in OVX rats.
研究大鼠卵巢切除不同时间后牙槽骨微结构的变化。
将24只未生育的Sprague-Dawley大鼠随机分为卵巢切除组（OVX）和假手术组（sham）。对OVX组大鼠进行双侧卵巢切除，假手术组大鼠进行假卵巢切除。术后3个月和6个月分别处死每组6只大鼠。对所有大鼠的右侧半上颌骨进行Micro-CT扫描，分析上颌第一磨牙根间牙槽骨情况。采用SPSS 16.0软件包进行统计分析。
牙槽骨的二维和三维重建图像显示，卵巢切除术后3个月和6个月大鼠均出现骨疏松改变，包括骨小梁变薄、变疏松以及骨髓腔扩大。与相应假手术组大鼠相比，卵巢切除术后3个月和6个月的OVX组大鼠牙槽骨的骨密度（BMD）、骨体积分数（BV/TV）和骨小梁厚度（Tb.Th）均显著降低（P<0.05）。两个OVX组的骨小梁数量（Tb.N）和骨小梁间距（Tb.Sp）均显著增加（P<0.05）。与卵巢切除术后3个月的大鼠相比，卵巢切除术后6个月的大鼠BMD、BV/TV和Tb.Th降低（P<0.05），Tb.N增加（P<0.05）。
OVX大鼠牙槽骨骨量丢失和骨小梁破坏随着卵巢切除时间延长而加重。
