Dai Q-G, Zhang P, Wu Y-Q, Ma X-H, Pang J, Jiang L-Y, Fang B
Department of Oral and Cranio-maxillofacial Science, Shanghai 9th People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Key Laboratory of Stomatology, Shanghai, China.
Oral Dis. 2014 Jul;20(5):514-20. doi: 10.1111/odi.12166. Epub 2013 Aug 6.
To investigate whether porotic changes occur in maxillary alveolar bone in ovariectomized rats.
Thirty-two 6-week-old female Sprague-Dawley rats were divided into an ovariectomy group (OVX) and a sham-ovariectomy group (sham). Twelve weeks after surgery, maxillae of 16 rats (eight OVX and eight sham) were analyzed by micro-CT. Inter-radicular alveolar bone of the maxillary first molar was reconstructed and analyzed. The remaining 16 maxillae were used for histochemistry, including hematoxylin-eosin staining and tartrate-resistant acid phosphatase enzyme staining.
Three-dimensional reconstructed images of the irregular alveolar bone showed an intuitive view of porotic changes in the OVX group. The alveolar bone in OVX rats had a porous microarchitecture including lower bone mass and a looser structure of more widely separated trabeculae. Bone mineral density (BMD), bone volume fraction (BV/TV), and trabecular thickness (Tb.Th.) were significantly lower in the OVX group than those in the sham group (P < 0.05). Trabecular separation (Tb.Sp.) increased significantly in the OVX rats compared to the sham rats (P < 0.05). Histomorphometric analysis of alveolar bone also revealed porotic changes in the OVX rats. Bone area ratio significantly decreased in the OVX group compared with the sham group (P < 0.01). There were also more osteoclasts present in the alveolar bone of OVX rats compared to sham rats (P < 0.05).
Ovariectomy induces osteoporosis in maxillary alveolar bone in rats, which may be related to the increased number of osteoclasts.
研究去卵巢大鼠上颌牙槽骨是否发生骨疏松改变。
将32只6周龄雌性Sprague-Dawley大鼠分为去卵巢组（OVX）和假去卵巢组（假手术组）。术后12周，对16只大鼠（8只OVX和8只假手术组）的上颌骨进行显微CT分析。重建并分析上颌第一磨牙的根间牙槽骨。其余16块上颌骨用于组织化学分析，包括苏木精-伊红染色和抗酒石酸酸性磷酸酶染色。
不规则牙槽骨的三维重建图像直观显示了OVX组的骨疏松改变。OVX大鼠的牙槽骨具有多孔微结构，包括骨量较低和小梁结构更疏松、间距更宽。OVX组的骨密度（BMD）、骨体积分数（BV/TV）和小梁厚度（Tb.Th.）显著低于假手术组（P<0.05）。与假手术组大鼠相比，OVX大鼠的小梁间距（Tb.Sp.）显著增加（P<0.05）。牙槽骨的组织形态计量学分析也显示OVX大鼠存在骨疏松改变。与假手术组相比，OVX组的骨面积比显著降低（P<0.01）。与假手术组大鼠相比，OVX大鼠牙槽骨中的破骨细胞也更多（P<0.05）。
去卵巢可导致大鼠上颌牙槽骨骨质疏松，这可能与破骨细胞数量增加有关。
