Ohuchi S, Hagiwara H, Ishido M, Fujita T, Kume T, Ishigaki I, Hirose S
Department of Biological Sciences, Tokyo Institute of Technology, Japan.
Biochem Biophys Res Commun. 1989 Jan 31;158(2):603-9. doi: 10.1016/s0006-291x(89)80092-0.
The interaction between the receptor (Rc) for atrial natriuretic peptide (ANP) and the effector enzyme particulate guanylate cyclase (GC) has been studied by radiation inactivation. Irradiation of bovine lung membranes produced an increase in GC activity at low radiation doses followed by a dose-dependent reduction at higher doses. This deviation from linearity in the inactivation curve disappeared when lung membranes were pretreated with ANP. Essentially identical results were also obtained with adrenal membranes. Based on these radiation inactivation data, the following dissociative mechanism of activation of particulate guanylate cyclase by ANP has been proposed: Rc.GC(inactive) + ANP----Rc.ANP + GC(active).
已通过辐射失活研究了心钠素（ANP）受体（Rc）与效应酶颗粒型鸟苷酸环化酶（GC）之间的相互作用。对牛肺膜进行辐射时，低辐射剂量下GC活性增加，而在较高剂量下则呈剂量依赖性降低。当肺膜用ANP预处理时，失活曲线中的这种线性偏差消失。肾上腺膜也得到了基本相同的结果。基于这些辐射失活数据，已提出以下ANP激活颗粒型鸟苷酸环化酶的解离机制：Rc.GC（无活性）+ ANP→Rc.ANP + GC（有活性）。
