Ishido M, Fujita T, Hagiwara H, Shimonaka M, Saheki T, Hirata Y, Hirose S
Biochem Biophys Res Commun. 1986 Oct 15;140(1):101-6. doi: 10.1016/0006-291x(86)91063-6.
Coupling of the atrial natriuretic peptide (ANP) receptor to particulate guanylate cyclase has been demonstrated kinetically and chromatographically using bovine lung plasma membranes and their detergent extracts. Addition of ANP to the membrane suspension stimulated guanylate cyclase activity 2-5-fold indicating the presence of ANP-sensitive particulate guanylate cyclase. The enzyme retained the ability to respond to ANP even after solubilization with digitonin. Characterization of the solubilized enzyme by gel filtration and affinity chromatography revealed that the ANP receptor and particulate guanylate cyclase exist as a functionally but not covalently linked stable complex.
利用牛肺质膜及其去污剂提取物，通过动力学和色谱法证明了心房利钠肽（ANP）受体与颗粒型鸟苷酸环化酶的偶联。向膜悬浮液中添加ANP可刺激鸟苷酸环化酶活性提高2至5倍，表明存在对ANP敏感的颗粒型鸟苷酸环化酶。即使在用洋地黄皂苷溶解后，该酶仍保留对ANP作出反应的能力。通过凝胶过滤和亲和色谱对溶解后的酶进行表征，结果显示ANP受体和颗粒型鸟苷酸环化酶以功能上而非共价连接的稳定复合物形式存在。
