Taylor Justin J, Pape Kathryn A, Steach Holly R, Jenkins Marc K
Department of Microbiology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455, USA. Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Research Center, Seattle, WA 98019, USA.
Department of Microbiology, Center for Immunology, University of Minnesota Medical School, Minneapolis, MN 55455, USA.
Science. 2015 Feb 13;347(6223):784-7. doi: 10.1126/science.aaa1342. Epub 2015 Jan 29.
When exposed to antigens, naïve B cells differentiate into different types of effector cells: antibody-producing plasma cells, germinal center cells, or memory cells. Whether an individual naïve B cell can produce all of these different cell fates remains unclear. Using a limiting dilution approach, we found that many individual naïve B cells produced only one type of effector cell subset, whereas others produced all subsets. The capacity to differentiate into multiple subsets was a characteristic of clonal populations that divided many times and resisted apoptosis, but was independent of isotype switching. Antigen receptor affinity also influenced effector cell differentiation. These findings suggest that diverse effector cell types arise in the primary immune response as a result of heterogeneity in responses by individual naïve B cells.
当暴露于抗原时，未成熟B细胞会分化为不同类型的效应细胞：产生抗体的浆细胞、生发中心细胞或记忆细胞。单个未成熟B细胞是否能够产生所有这些不同的细胞命运仍不清楚。使用有限稀释法，我们发现许多单个未成熟B细胞仅产生一种效应细胞亚群，而其他细胞则产生所有亚群。分化为多个亚群的能力是多次分裂并抵抗凋亡的克隆群体的特征，但与同种型转换无关。抗原受体亲和力也影响效应细胞的分化。这些发现表明，在初次免疫反应中，由于单个未成熟B细胞反应的异质性，会产生多种不同类型的效应细胞。
