Holland R E, Sriranganathan N, DuPont L
Department of Large Animal Clinical Sciences, Virginia-Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg 24061.
J Am Vet Med Assoc. 1989 Feb 1;194(3):389-91.
Enterotoxigenic Escherichia coli was isolated from a 3-day-old foal with diarrhea. The isolate was distinguished from nonpathogenic E coli by determining the presence of pili and enterotoxin production. A standard slide agglutination test was performed, using pooled antisera that contained antibodies against K99 and F41 pilus antigens, K87 capsular antigen, and 0101 somatic antigen. Agglutination of the antisera occurred in the presence of the isolate. Piliation was verified by use of negative-contrast electron microscopy. Further, the isolate produced a heat-labile enterotoxin-like antigen that cross-reacted with a reagent containing formalin-treated, heat-killed Staphylococcus aureus (cowan 1 strain) bearing anti-cholera antibodies. On the basis of the aforementioned procedures and the absence of other identifiable enteric pathogens, we believe that E coli was responsible for causing diarrhea in the foal.
从一匹3日龄患腹泻的马驹中分离出产肠毒素大肠杆菌。通过检测菌毛的存在和肠毒素的产生，将该分离株与非致病性大肠杆菌区分开来。使用含有抗K99和F41菌毛抗原、K87荚膜抗原以及0101菌体抗原抗体的混合抗血清进行标准玻片凝集试验。在有该分离株存在的情况下，抗血清发生凝集。通过负染色电子显微镜证实了菌毛的存在。此外，该分离株产生了一种热不稳定的肠毒素样抗原，它与含有经福尔马林处理、热灭活的带有抗霍乱抗体的金黄色葡萄球菌（考恩1菌株）的试剂发生交叉反应。基于上述程序以及未发现其他可识别的肠道病原体，我们认为大肠杆菌是导致马驹腹泻的原因。
