Olivo Giovane, Lucas Thays Mizuki, Borges Alexandre Secorun, Silva Rodrigo Otávio Silveira, Lobato Francisco Carlos Faria, Siqueira Amanda Keller, da Silva Leite Domingos, Brandão Paulo Eduardo, Gregori Fábio, de Oliveira-Filho José Paes, Takai Shinji, Ribeiro Márcio Garcia
Department of Veterinary Clinical Sciences, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brazil.
Department of Veterinary Hygiene and Public Health, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brazil.
Biomed Res Int. 2016;2016:1512690. doi: 10.1155/2016/1512690. Epub 2016 Dec 27.
Diarrhea is a major clinical problem affecting foals up to 3 months of age. The aim of this study was to identify enteric microorganisms involved in monoinfections and coinfections and the associated virulence factors in healthy and diarrheic foals. Diarrheic (D) ( = 56) and nondiarrheic (ND) foals ( = 60) up to three months of age were studied. Fecal samples were analyzed for identification of infectious agents (microbiological culturing, molecular techniques, and microscopic analyses). (30% versus 25%), spp. (25% versus 7%), (25% versus 25%), type A (21% versus 10%), (20% versus 35%), (11% versus 18%), and -positive (5% versus 2%) were the most frequent enteric pathogens detected in D and ND foals, respectively. The frequency of toxin A-positive was significantly increased in the D ( = 0.033) compared with the ND animals. strains harboring virulent plasmids were also identified (VapA 85-kb type I and VapA 87-kb type I) in D and ND foals. Coinfections were observed in 46% of the D and 33% of the ND foals. Our results demonstrate the great diversity of enteric pathogens, virulence factors, and coinfections involved in enteric infections of foals.
腹泻是影响3月龄内马驹的一个主要临床问题。本研究的目的是确定健康和腹泻马驹单一感染及混合感染中涉及的肠道微生物及其相关毒力因子。对56匹腹泻（D）马驹和60匹非腹泻（ND）马驹（均为3月龄内）进行了研究。对粪便样本进行分析以鉴定感染因子（微生物培养、分子技术和显微镜分析）。在腹泻和非腹泻马驹中分别检测到的最常见肠道病原体为：（30%对25%）、 spp.（25%对7%）、（25%对25%）、A型（21%对10%）、（20%对35%）、（11%对18%）以及 -阳性（5%对2%）。与非腹泻动物相比，腹泻马驹中毒素A阳性的频率显著增加（ = 0.033）。在腹泻和非腹泻马驹中还鉴定出携带毒性质粒的菌株（I型85 kb VapA和I型87 kb VapA）。46%的腹泻马驹和33%的非腹泻马驹存在混合感染。我们的结果表明，马驹肠道感染中涉及的肠道病原体、毒力因子和混合感染具有高度多样性。
