Butts Ian Anthony Ernest, Baeza Rosa, Støttrup Josianne Gatt, Krüger-Johnsen Maria, Jacobsen Charlotte, Pérez Luz, Asturiano Juan F, Tomkiewicz Jonna
Technical University of Denmark, National Institute of Aquatic Resources, Jægersborg Allé 1, 2920 Charlottenlund, Denmark.
Grupo de Acuicultura y Biodiversidad, Instituto de Ciencia y Tecnología Animal, Universitat Politècnica de València, 46022 Valencia, Spain.
Comp Biochem Physiol A Mol Integr Physiol. 2015 May;183:87-96. doi: 10.1016/j.cbpa.2015.01.015. Epub 2015 Jan 29.
In order for European eel aquaculture to be sustainable, the life cycle should be completed in captivity. Development of broodstock diets may improve the species' reproductive success in captivity, through the production of high-quality gametes. Here, our aim was to evaluate the influence of dietary regime on muscle composition, and liver lipids prior to induced maturation, and the resulting sperm composition and performance. To accomplish this fish were reared on three "enhanced" diets and one commercial diet, each with different levels of fatty acids, arachidonic acid (ARA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA). Neutral lipids from the muscle and liver incorporated the majority of the fatty acid profile, while phospholipids incorporated only certain fatty acids. Diet had an effect on the majority of sperm fatty acids, on the total volume of extractable milt, and on the percentage of motile sperm. Here, our results suggest that the total volume of extractable milt is a DHA-dependent process, as we found the diets with the highest DHA levels induced the most milt while the diet with the lowest DHA level induced the least amount of milt. The diet with the highest level of ARA induced medium milt volumes but had the highest sperm motility. EPA also seems important for sperm quality parameters since diets with higher EPA percentages had a higher volume of milt and higher sperm motility. In conclusion, dietary fatty acids had an influence on fatty acids in the tissues of male eel and this impacted sperm performance.
为使欧洲鳗鱼养殖可持续发展，其生命周期应在圈养环境中完成。亲鱼饲料的研发或许可通过产生高质量配子来提高该物种在圈养环境中的繁殖成功率。在此，我们的目的是评估在诱导成熟前饮食方案对肌肉组成、肝脏脂质的影响，以及由此产生的精子组成和性能。为实现这一目标，将鱼饲养在三种“强化”饲料和一种商业饲料上，每种饲料的脂肪酸、花生四烯酸（ARA）、二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）水平各不相同。肌肉和肝脏中的中性脂质包含了大部分脂肪酸谱，而磷脂仅包含某些脂肪酸。饲料对大多数精子脂肪酸、可提取精液的总体积以及活动精子的百分比都有影响。在此，我们的结果表明，可提取精液的总体积是一个依赖DHA的过程，因为我们发现DHA水平最高的饲料诱导产生的精液最多，而DHA水平最低的饲料诱导产生的精液最少。ARA水平最高的饲料诱导产生中等体积的精液，但精子活力最高。EPA对精子质量参数似乎也很重要，因为EPA百分比更高的饲料产生的精液体积更大，精子活力更高。总之，膳食脂肪酸对雄性鳗鱼组织中的脂肪酸有影响，进而影响精子性能。
