Frazão M R, Falcão J P
Brazilian Reference Center on Yersinia spp. other than Y. pestis, Departamento de Análises Clínicas, Toxicológicas e Bromatológicas, Faculdade de Ciências Farmacêuticas de Ribeirão Preto - USP, Ribeirão Preto, Brazil.
J Appl Microbiol. 2015 Apr;118(4):1058-67. doi: 10.1111/jam.12751. Epub 2015 Feb 8.
To investigate the pathogenic potential and genotypic diversity of Yersinia enterocolitica biotype 2 strains isolated in Brazil and to compare these strains with other Y. enterocolitica biotypes using ERIC-PCR and PFGE.
Forty strains of Y. enterocolitica biotype 2 (B2) isolated from humans (5), the environment (34) and animal (1), in Brazil over 19 years were studied. In addition to these isolates, we also analysed 26 Y. enterocolitica strains belonging to the biotypes 1A, 1B, and 3-5. All of the B2 strains contained the genes inv, ail, ystA, hreP, tccC and myfA. The genes fepD and fes were detected in 39 (97·5%) strains, virF was found in three (7·5%) strains, and ystB and fepA were not detected in any strains. The B2 strains showed genotypic similarities of more than 84·8% by ERIC-PCR and of more than 69·0% by PFGE.
The pathogenic potential of the B2 strains examined in this study was highlighted by the occurrence of the majority of the virulence markers searched. The results of the ERIC-PCR and PFGE showed that the B2 strains evaluated in this study had a high genotypic similarity, suggesting that these strains differed little over the 19 year study period and that the environment was a possible source of contamination of humans and animals in Brazil. Furthermore, the ERIC-PCR technique grouped the strains belonging to Y. enterocolitica biotypes 1A, 1B, 2, 3, 4 and 5 according to their pathogenicity.
This study provided new information about the pathogenic potential and genotypic similarity of Y. enterocolitica B2 isolated from diverse sources in Brazil. Furthermore, ERIC-PCR showed to be a valuable tool for grouping Y. enterocolitica of different biotypes according their pathogenicity.
研究在巴西分离出的小肠结肠炎耶尔森菌生物2型菌株的致病潜力和基因多样性，并使用ERIC-PCR和PFGE技术将这些菌株与其他小肠结肠炎耶尔森菌生物型进行比较。
对19年间在巴西从人类（5株）、环境（34株）和动物（1株）中分离出的40株小肠结肠炎耶尔森菌生物2型（B2）进行了研究。除了这些分离株外，我们还分析了26株属于生物1A、1B和3-5型的小肠结肠炎耶尔森菌菌株。所有B2菌株都含有inv、ail、ystA、hreP、tccC和myfA基因。在39株（97.5%）菌株中检测到fepD和fes基因，在3株（7.5%）菌株中发现virF基因，在任何菌株中均未检测到ystB和fepA基因。通过ERIC-PCR，B2菌株显示出超过84.8%的基因相似性，通过PFGE显示出超过69.0%的基因相似性。
本研究中检测的B2菌株的致病潜力通过所搜索的大多数毒力标记的出现得到凸显。ERIC-PCR和PFGE的结果表明，本研究中评估的B2菌株具有高度的基因相似性，表明这些菌株在19年的研究期间变化不大，并且环境可能是巴西人类和动物污染的一个来源。此外，ERIC-PCR技术根据致病性将属于小肠结肠炎耶尔森菌生物1A、1B、2、3、4和5型的菌株进行了分组。
本研究提供了关于从巴西不同来源分离出的小肠结肠炎耶尔森菌B2的致病潜力和基因相似性的新信息。此外，ERIC-PCR显示是根据致病性对不同生物型的小肠结肠炎耶尔森菌进行分组的有价值工具。
