Department of Veterinary Medicine and Animal Production, University of Naples "Federico II", Via Delpino 1, 80137 Napoli, Italy.
Department of Food Microbiology, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute, 2, 80055 Portici, NA, Italy.
Food Microbiol. 2017 Aug;65:231-235. doi: 10.1016/j.fm.2017.03.004. Epub 2017 Mar 6.
Yersinia enterocolitica comprises six biotypes 1A, 1B, 2, 3, 4, and 5. The virulence of the strains belonging to biotypes 1B and 2-5 depends on the presence of both chromosomal and plasmid-borne genes. Strains belonging to biotype 1A do not carry the virulence plasmid pYV. However, they carry other virulence genes, such as ystB and hreP. The aim of this study was to investigate the distribution of yadA, virF, inv, ystA, ystB, myfA, hreP and ymoA virulence genes in Y. enterocolitica strains in order to select the target genes that could be used for the development of a probe-specific real-time PCR to determine the presence of Y. enterocolitica in food samples. A total of 161 Y. enterocolitica strains isolated in eight countries and grouped into biotypes 1A, 2 (serotypes O3, O5 and O9), 3 (serotypes O3 and O9) and 4 (serotype O3) were examined for virulence genes. The most common virulence-associated gene in pathogenic Y. enterocolitica proved to be ystA, which can therefore be considered the best target gene to be amplified in order to evaluate the presence of pathogenic biotypes. By contrast, to identify Y. enterocolitica 1A strains, ystB, which codes for the enterotoxin YstB, can be proposed. This has been found in all non-pathogenic biotypes studied, but never in pathogenic biotypes.
小肠结肠炎耶尔森菌包含 6 个生物型 1A、1B、2、3、4 和 5。属于生物型 1B 和 2-5 的菌株的毒力取决于染色体和质粒携带基因的存在。属于生物型 1A 的菌株不携带毒力质粒 pYV。然而，它们携带其他毒力基因，如 ystB 和 hreP。本研究旨在调查 yadA、virF、inv、ystA、ystB、myfA、hreP 和 ymoA 毒力基因在小肠结肠炎耶尔森菌菌株中的分布，以选择可用于探针特异性实时 PCR 开发的目标基因，以确定食品样本中是否存在小肠结肠炎耶尔森菌。总共检查了 161 株从 8 个国家分离的小肠结肠炎耶尔森菌菌株，分为生物型 1A、2（血清型 O3、O5 和 O9）、3（血清型 O3 和 O9）和 4（血清型 O3），并对其进行了毒力基因检测。在致病性小肠结肠炎耶尔森菌中最常见的与毒力相关的基因是 ystA，因此可以考虑将其作为扩增的最佳目标基因，以评估致病性生物型的存在。相比之下，为了鉴定小肠结肠炎耶尔森菌 1A 菌株，可以提出 ystB，它编码肠毒素 YstB。该基因存在于所有研究的非致病性生物型中，但从未存在于致病性生物型中。
