Faculty of Medicine, University of Coimbra and Obstetric Unit, Coimbra University Hospital Centre, Coimbra, Portugal.
Faculty of Medicine, University of Coimbra, Coimbra, Portugal.
BJOG. 2015 Oct;122(11):1544-50. doi: 10.1111/1471-0528.13294. Epub 2015 Feb 2.
To provide evidence of the existence of membrane progesterone receptor alpha (mPRα) on regulatory T cells (Treg) in peripheral blood during pregnancy, postulating a possible explanation for the effect of progesterone on preterm birth.
Cross-sectional study.
Tertiary Obstetric Department in a University Hospital.
Healthy pregnant women.
Treg cells from peripheral blood samples were studied by flow cytometry using multiple monoclonal antibody expression.
Evaluate the number and percentage of CD4(+) CD25(high) CD127(low) , the number and percentage of Treg cells among the total CD4(+) T cells, and the percentage and mean fluorescence intensity (MFI) of mPRα in that population, using several gating strategies.
43 peripheral blood samples were collected from healthy women during pregnancy, whose median gestational age was 28.7 ± 7.1 (16-40) weeks. The percentage of CD4(+) in the total lymphocytes was 43% (32-51) and the percentage of CD4(+) CD25(high) CD127(low) was 4.8% (1.6-5.9), with only 45% (16-72) of those cells expressing the intracellular marker FoxP3 (Treg cell pool). We confirmed the existence of mPRα in that specific population because 8.0% (2.02-33) of the Treg cells were marked with the specific monoclonal antibody, with an mPRα(+) MFI of 719 (590-1471).
This research shows that Treg cells express mPRα during pregnancy, which might play an important role in immune modulation by progesterone.
提供妊娠期间外周血调节性 T 细胞（Treg）上膜孕激素受体 alpha（mPRα）存在的证据，推测孕激素对早产的影响的可能解释。
横断面研究。
大学医院的三级产科病房。
健康孕妇。
使用多种单克隆抗体表达通过流式细胞术研究外周血样本中的 Treg 细胞。
评估 CD4(+) CD25(high) CD127(low) 的数量和百分比，Treg 细胞在总 CD4(+) T 细胞中的数量和百分比，以及该群体中 mPRα 的百分比和平均荧光强度（MFI），使用几种门控策略。
从妊娠期间的 43 名健康女性采集了外周血样本，其中位妊娠年龄为 28.7 ± 7.1（16-40）周。总淋巴细胞中 CD4(+)的百分比为 43%（32-51），CD4(+) CD25(high) CD127(low)的百分比为 4.8%（1.6-5.9），只有 45%（16-72）的细胞表达细胞内标志物 FoxP3（Treg 细胞池）。我们证实了特定群体中 mPRα 的存在，因为 8.0%（2.02-33）的 Treg 细胞被特异性单克隆抗体标记，mPRα(+) MFI 为 719（590-1471）。
这项研究表明，Treg 细胞在妊娠期间表达 mPRα，这可能在孕激素的免疫调节中发挥重要作用。
