Jiang Jiayang, Zhang Chuanqi, Lin Wenpeng, Liu Yahong, Liu Shujuan, Xu Yunjian, Zhao Qiang, Huang Wei
Key Laboratory for Organic Electronics and Information Displays & Institute of Advanced Materials (IAM), Jiangsu National Synergistic Innovation Center for Advanced Materials (SICAM), Nanjing University of Posts & Telecommunications (NUPT), Nanjing, 210023, P.R. China.
Macromol Rapid Commun. 2015 Apr;36(7):640-6. doi: 10.1002/marc.201400654. Epub 2015 Jan 13.
The applications of conjugated polyelectrolytes in biosensing and bioimaging have attracted more and more research interests due to their excellent photophysical properties. In this work, a new series of conjugated polyelectrolytes containing long-lived phosphorescent Ir(III) complexes is designed and synthesized, which can be used for ratiometric and lifetime-based sensing of heparin utilizing the electrostatic interaction between cationic polymers and anionic heparin. By changing the ligand structures of Ir(III) complexes, the sensing performances of phosphorescent-conjugated polyelectrolytes (PCPEs) are optimized. In addition, the application of PCPEs in cellular imaging is carried out. These polymers can be applied for specific staining of cell membrane. Importantly, utilizing the long emission lifetime of phosphorescent signal of Ir(III) complexes, time-gated luminescent imaging is carried out, which can eliminate the short-lived background fluorescence interferences from the environment or biological samples.
由于其优异的光物理性质，共轭聚电解质在生物传感和生物成像中的应用已吸引了越来越多的研究兴趣。在这项工作中，设计并合成了一系列含有长寿命磷光铱（III）配合物的新型共轭聚电解质，其可利用阳离子聚合物与阴离子肝素之间的静电相互作用用于肝素的比率和基于寿命的传感。通过改变铱（III）配合物的配体结构，优化了磷光共轭聚电解质（PCPEs）的传感性能。此外，还开展了PCPEs在细胞成像中的应用。这些聚合物可用于细胞膜的特异性染色。重要的是，利用铱（III）配合物磷光信号的长发射寿命，进行了时间门控发光成像，这可以消除来自环境或生物样品的短寿命背景荧光干扰。
