Bree Dara, Moriarty Orla, O'Mahony Cliona M, Morris Bradley, Bannerton Karen, Broom Daniel C, Kelly John P, Roche Michelle, Finn David P
Discipline of Pharmacology and Therapeutics, National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland; Discipline of Physiology, School of Medicine, National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland; Galway Neuroscience Centre and Centre for Pain Research, National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland.
Discipline of Pharmacology and Therapeutics, National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland; Discipline of Physiology, School of Medicine, National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland; Galway Neuroscience Centre and Centre for Pain Research, National Centre for Biomedical Engineering Science, National University of Ireland, Galway, Ireland; Research and Development, Covidien, North Haven, Connecticut.
J Pain. 2015 May;16(5):421-35.e1-6. doi: 10.1016/j.jpain.2015.01.010. Epub 2015 Jan 30.
Acute postoperative pain remains a significant health care issue. Development of anatomically relevant animal models of postoperative pain, with improved predictive validity, would advance understanding of postoperative pain mechanisms and improve treatment outcomes. This study aimed to develop, characterize, and validate a rat model of acute postoperative pain associated with inguinal hernia repair based on the Lichtenstein inguinal hernia repair procedure (without hernia induction). We hypothesized that the surgery would result in reduced spontaneous locomotor activity, which would represent a pain-related phenotype. Postsurgical characterization involved extensive monitoring of home cage and open field locomotor activity, as well as mechanical hypersensitivity and assessment of c-Fos expression in the dorsal horn of the spinal cord. In pharmacologic validation studies, rats received morphine, carprofen, or paracetamol 1 hour before, and/or immediately after, surgery. Rats that underwent hernia repair surgery exhibited significantly lower horizontal and vertical activities in the home cage and open field in the early postsurgical period, compared with sham rats or rats that underwent skin incision only. Morphine, carprofen, and paracetamol attenuated the surgery-induced reductions in locomotor activity, to varying degrees. Surgery was associated with significantly increased c-Fos expression in the ipsilateral dorsal horn of the spinal cord, an effect attenuated by carprofen treatment. These results support the development and characterization of a novel, anatomically relevant animal model of acute postoperative pain that may facilitate development of improved treatment regimens.
Acute pain following inguinal hernia repair can be difficult to treat. Here we report, for the first time, the development of a novel, anatomically relevant rat model to facilitate improved understanding and treatment of acute postoperative pain following inguinal hernia repair.
急性术后疼痛仍然是一个重大的医疗保健问题。开发具有更高预测效度的与术后疼痛相关的解剖学相关动物模型，将有助于推进对术后疼痛机制的理解并改善治疗效果。本研究旨在基于Lichtenstein腹股沟疝修补术（不诱导疝形成）开发、表征并验证一种与腹股沟疝修补相关的急性术后疼痛大鼠模型。我们假设该手术会导致自发运动活动减少，这将代表一种与疼痛相关的表型。术后表征包括对饲养笼和旷场运动活动的广泛监测，以及机械性超敏反应和脊髓背角c-Fos表达的评估。在药理学验证研究中，大鼠在手术前1小时和/或手术后立即接受吗啡、卡洛芬或对乙酰氨基酚。与假手术大鼠或仅接受皮肤切口的大鼠相比，接受疝修补手术的大鼠在术后早期饲养笼和旷场中的水平和垂直活动显著降低。吗啡、卡洛芬和对乙酰氨基酚在不同程度上减轻了手术引起的运动活动减少。手术与脊髓同侧背角c-Fos表达显著增加相关，卡洛芬治疗可减弱这种效应。这些结果支持开发和表征一种新型的、与解剖学相关的急性术后疼痛动物模型，这可能有助于开发更好的治疗方案。
腹股沟疝修补术后的急性疼痛可能难以治疗。在此我们首次报告开发了一种新型的、与解剖学相关的大鼠模型，以促进对腹股沟疝修补术后急性术后疼痛的更好理解和治疗。
