Brazão-Silva Marco T, Rodrigues Maria Fernandes S, Eisenberg Ana Lúcia A, Dias Fernando L, de Castro Luciana M, Nunes Fábio D, Faria Paulo R, Cardoso Sérgio V, Loyola Adriano M, de Sousa Suzana C O M
PhD program in Estomatology and Basic and Applied Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil.
Laboratory of Molecular Pathology, School of Dentistry, University of São Paulo, São Paulo, Brazil.
Histopathology. 2015 Sep;67(3):358-67. doi: 10.1111/his.12660. Epub 2015 Mar 8.
Metallothioneins (MTs) are proteins associated with the carcinogenesis and prognosis of various tumours. Previous studies have shown their potential as biomarkers in oral squamous cell carcinoma (OSCC). Aiming to understand more clearly the function of MTs in OSCC we evaluated, for the first time, the gene expression profile of MTs in this neoplasm.
Tissue samples from 35 cases of tongue and/or floor of mouth OSCC, paired with their corresponding non-neoplastic oral mucosa (NNOM), were retrieved (2007-09). All tissues were analysed for the following genes using TaqMan(®) reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays: MT1A, MT1B, MT1E, MT1F, MT1G, MT1H, MT1X, MT2A, MT3 and MT4. The expression of MT1B and MT1H was seldom detected in both OSCC and NNOM. A significant loss of MT1A, MT1X, MT3 and MT4 expression and gain of MT1F expression was observed in OSCC, compared to NNOM. Cases with MT1G down-regulation exhibited the worst prognoses. The up-regulation of MT1X was restricted to non-metastatic cases, whereas up-regulation of MT3 was related to cases with lymph node metastasis.
Metallothionein mRNA expression is altered significantly in oral squamous cell carcinomas. The expression of MT1G, MT1X and MT3 may aid in the prognostic discrimination of OSCC cases.
金属硫蛋白（MTs）是与各种肿瘤的发生和预后相关的蛋白质。先前的研究表明它们在口腔鳞状细胞癌（OSCC）中作为生物标志物的潜力。为了更清楚地了解MTs在OSCC中的功能，我们首次评估了该肿瘤中MTs的基因表达谱。
收集了35例舌和/或口底OSCC的组织样本及其相应的非肿瘤性口腔黏膜（NNOM）（2007 - 2009年）。使用TaqMan®逆转录定量聚合酶链反应（RT - qPCR）分析对所有组织的以下基因进行检测：MT1A、MT1B、MT1E、MT1F、MT1G、MT1H、MT1X、MT2A、MT3和MT4。在OSCC和NNOM中很少检测到MT1B和MT1H的表达。与NNOM相比，OSCC中观察到MT1A、MT1X、MT3和MT4表达显著降低以及MT1F表达增加。MT1G下调的病例预后最差。MT1X的上调仅限于非转移病例，而MT3的上调与淋巴结转移病例相关。
口腔鳞状细胞癌中金属硫蛋白mRNA表达有显著改变。MT1G、MT1X和MT3的表达可能有助于OSCC病例的预后鉴别。
