金属硫蛋白异构体在甲状腺良恶性病变中的表达

Metallothionein Isoform Expression in Benign and Malignant Thyroid Lesions.

作者信息

Wojtczak Beata, Pula Bartosz, Gomulkiewicz Agnieszka, Olbromski Mateusz, Podhorska-Okolow Marzena, Domoslawski Paweł, Bolanowski Marek, Daroszewski Jacek, Dziegiel Piotr

机构信息

First Department and Clinic of General, Gastroenterological and Endocrine Surgery, Wroclaw Medical University, Wroclaw, Poland

Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland.

出版信息

Anticancer Res. 2017 Sep;37(9):5179-5185. doi: 10.21873/anticanres.11940.

DOI:10.21873/anticanres.11940

PMID:28870952

Abstract

BACKGROUND

Metallothioneins (MTs) are involved in numerous cell processes such as binding and transport of zinc and copper ions, differentiation, proliferation and apoptosis, therefore contributing to carcinogenesis. Scarce data exist on their expression in benign and malignant lesions of the thyroid.

MATERIALS AND METHODS

mRNA expression of functional isoforms of MT genes (MT1A, MT1B, MT1E, MT1F, MT1G, MT1H, MT1X, MT2A, MT4) was studied in 17 nodular goiters (NG), 12 follicular adenomas (FA) and 26 papillary thyroid carcinomas (PTC).

RESULTS

One-way ANOVA revealed significant differences in mRNA expression levels of MT1A (p<0.05), MT1E (p<0.005), MT1F (p<0.0001), MT1G (p<0.005), MT1X (p<0.0005) and MT2A (p<0.005) in the analyzed samples. Post hoc analysis confirmed a significantly lower expression of MT1A mRNA in PTC compared to NG (p<0.05). Significant down-regulation was also noted for other MT isoforms in PTC in comparison to NG: MT1E (p<0.05), MT1F (p<0.0001), MT1G (p<0.005), MT1X (p<0.0005) and MT2A (p<0.05). In addition, significant down-regulation of MT1F and MT1G in FA compared to NG was observed (p<0.005 and p<0.05, respectively).

CONCLUSION

Expression of functional MT isoforms may contribute to thyroid carcinogenesis and potentially serve as a diagnostic marker in distinguishing benign and malignant lesions.

摘要

背景

金属硫蛋白（MTs）参与众多细胞过程，如锌和铜离子的结合与转运、分化、增殖和凋亡，因此与癌症发生有关。关于它们在甲状腺良恶性病变中的表达数据稀少。

材料与方法

研究了MT基因功能异构体（MT1A、MT1B、MT1E、MT1F、MT1G、MT1H、MT1X、MT2A、MT4）的mRNA表达，样本包括17例结节性甲状腺肿（NG）、12例滤泡性腺瘤（FA）和26例甲状腺乳头状癌（PTC）。

结果

单因素方差分析显示，分析样本中MT1A（p<0.05）、MT1E（p<0.005）、MT1F（p<0.0001）、MTG（p<0.005）、MT1X（p<0.0005）和MT2A（p<0.005）的mRNA表达水平存在显著差异。事后分析证实，与NG相比，PTC中MT1A mRNA的表达显著降低（p<0.05）。与NG相比，PTC中其他MT异构体也有显著下调：MT1E（p<0.05）、MT1F（p<0.0001）、MT1G（p<0.005）、MT1X（p<0.0005）和MT2A（p<0.05）。此外，与NG相比，FA中MT1F和MT1G也有显著下调（分别为p<0.005和p<0.05）。