Wang Yuan Min, Wang Yiping, Harris David C H, Alexander Stephen I, Lee Vincent W S
Centre for Kidney Research, Children's Hospital at Westmead, Sydney, Australia.
Centre for Transplant and Renal Research, Westmead Millennium Institute, University of Sydney at Westmead Hospital, Sydney, Australia.
Curr Protoc Immunol. 2015 Feb 2;108:15.28.1-15.28.6. doi: 10.1002/0471142735.im1528s108.
Chronic proteinuric renal injury is a major cause of end stage renal disease. Adriamycin nephropathy (AN) is a murine model of chronic proteinuric renal disease whereby chemical injury is followed by immune and structural changes that mimic human disease. This unit describes the method of AN induced by a single injection of adriamycin (ADR) in BALB/c mice. After the initial toxic injury, an immune-mediated chronic proteinuric renal disease that resembles human focal segmental glomerulosclerosis develops. The clinic pathological features of AN are nephrotic syndrome, focal glomerulosclerosis, tubular injury, and interstitial compartment expansion with mononuclear cell infiltrates that are composed largely of macrophages and T cells.
慢性蛋白尿性肾损伤是终末期肾病的主要原因。阿霉素肾病（AN）是一种慢性蛋白尿性肾病的小鼠模型，化学损伤后会出现免疫和结构变化，类似于人类疾病。本单元描述了在BALB/c小鼠中单次注射阿霉素（ADR）诱导AN的方法。在最初的毒性损伤后，会发展出一种类似于人类局灶节段性肾小球硬化的免疫介导的慢性蛋白尿性肾病。AN的临床病理特征为肾病综合征、局灶性肾小球硬化、肾小管损伤以及间质区扩张，伴有主要由巨噬细胞和T细胞组成的单核细胞浸润。
