Leibig Nico, Boyle Veronika, Kraus Daniel, Stark Gerhard Bjoern, Penna Vincenzo
From the *Clinic of Hand, Plastic and Reconstructive Surgery, Burn Center, BG Trauma Center, Ludwigshafen; †Clinic for Neurology, Ortenau Klinikum Lahr-Ettenheim, Lahr; and ‡Clinic of Plastic and Hand Surgery, University Medical Center Freiburg, Freiburg, Germany.
Ann Plast Surg. 2015 Mar;74(3):350-3. doi: 10.1097/SAP.0000000000000415.
Peripheral nerve regeneration over longer distances through conduits is limited. In the presented study, critical size nerve gap bridging with a poly-DL-lactide-ε-caprolactone (PLC) conduit was combined with application of C3 toxin to facilitate axonal sprouting.
The PLC filled with fibrin (n = 10) and fibrin gel loaded with 1-μg C3-C2I and 2-μg C2II (n = 10) were compared to autologous nerve grafts (n = 10) in a 15-mm sciatic nerve gap lesion model of the rat. Functional and electrophysiological analyses were performed before histological evaluation.
Evaluation of motor function and nerve conduction velocity at 16 weeks revealed no differences between the groups. All histological parameters and muscle weight were significantly elevated in nerve graft group. No differences were observed in both PLC groups.
The PLCs are permissive for nerve regeneration over a 15-mm defect in rats. Intraluminal application of C3 toxin did not lead to significant enhancement of nerve sprouting.
通过导管进行长距离外周神经再生是有限的。在本研究中，将聚-DL-丙交酯-ε-己内酯（PLC）导管桥接临界尺寸神经缺损与应用C3毒素相结合，以促进轴突萌发。
在大鼠15毫米坐骨神经缺损损伤模型中，将填充有纤维蛋白的PLC（n = 10）和负载1μg C3-C2I和2μg C2II的纤维蛋白凝胶（n = 10）与自体神经移植（n = 10）进行比较。在组织学评估之前进行功能和电生理分析。
16周时运动功能和神经传导速度评估显示各组之间无差异。神经移植组的所有组织学参数和肌肉重量均显著升高。两个PLC组均未观察到差异。
PLC允许大鼠15毫米缺损处的神经再生。管腔内应用C3毒素未导致神经萌发的显著增强。
