Nikolaev S I, Gallyamov A R, Mamin G V, Chelyshev Yu A
Department of Histology, Cytology, and Embryology, Kazan State Medical University, Kazan, Russia.
Bull Exp Biol Med. 2014 May;157(1):155-8. doi: 10.1007/s10517-014-2513-1. Epub 2014 Jun 11.
We studied regeneration of rat sciatic nerve while overcoming of a 5-mm diastasis with the aid of nanostructured conduit made of biocompatible and biodegradable poly(ε-caprolactone) and filled with fibrin hydrogel matrix. Implantation of the conduit into the nerve in combination with local delivery of the expression plasmid carrying genes encoding vascular endothelial growth factor (vegf) and fibroblast growth factor 2 (fgf2) leads to an increase in number of myelinated fibers and S-100(+) cells in the peripheral nerve stump and improved recovery of the nerve function. Under conditions of direct gene therapy, an advantage of electrospun poly(ε-caprolactone) conduit with high-porosity was revealed on the basis of these criteria in comparison with biocompatible silicon conduit.
我们研究了大鼠坐骨神经的再生情况，在此过程中借助由生物相容性和可生物降解的聚（ε-己内酯）制成并填充有纤维蛋白水凝胶基质的纳米结构导管，克服了5毫米的神经间隙。将该导管植入神经并结合局部递送携带编码血管内皮生长因子（VEGF）和成纤维细胞生长因子2（FGF2）的基因的表达质粒，可导致周围神经残端中有髓纤维和S-100（+）细胞数量增加，并改善神经功能的恢复。在直接基因治疗的条件下，基于这些标准，与生物相容性硅导管相比，具有高孔隙率的电纺聚（ε-己内酯）导管显示出优势。
