将基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）检测碳青霉烯酶活性应用于肠杆菌科细菌、铜绿假单胞菌和鲍曼不动杆菌临床分离株，并与Carba-NP检测法进行比较。
MALDI-TOF MS detection of carbapenemase activity in clinical isolates of Enterobacteriaceae spp., Pseudomonas aeruginosa, and Acinetobacter baumannii compared against the Carba-NP assay.
作者信息
Chong Patrick M, McCorrister Stuart J, Unger Mark S, Boyd David A, Mulvey Michael R, Westmacott Garrett R
机构信息
Mass Spectrometry and Proteomics Core, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
Antimicrobial Resistance and Nosocomial Infections, National Microbiology Laboratory, Public Health Agency of Canada, 1015 Arlington Street, Winnipeg, Manitoba R3E 3R2, Canada.
出版信息
J Microbiol Methods. 2015 Apr;111:21-3. doi: 10.1016/j.mimet.2015.01.024. Epub 2015 Jan 30.
MALDI-TOF MS detection of carbapenemase-activity in Gram-negative bacteria was compared against the Carba-NP assay. MALDI-TOF MS detected activity from 99% of the strains, from all types of carbapenemase (200/202), while Carba-NP assays detected activity from 85% (45/53) of the tested isolates and could not consistently identify OXA- or GES carbapenemase activity.
将革兰氏阴性菌中碳青霉烯酶活性的基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）检测结果与碳青霉烯酶显色试验（Carba-NP试验）进行比较。MALDI-TOF MS检测出所有类型碳青霉烯酶（202株中的200株）中99%菌株的活性，而Carba-NP试验检测出85%（53株中的45株）受试分离株的活性，且无法始终如一地鉴定出OXA或GES碳青霉烯酶活性。
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