Hegazy Maha A, Abdelwahab Nada S, Fayed Ahmed S
Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Egypt.
Analytical Chemistry Department, Faculty of Pharmacy, Benisuef University, Egypt.
Spectrochim Acta A Mol Biomol Spectrosc. 2015 Apr 5;140:524-33. doi: 10.1016/j.saa.2014.12.108. Epub 2015 Jan 20.
A novel method was developed for spectral resolution and further determination of five-component mixture including Vitamin B complex (B1, B6, B12 and Benfotiamine) along with the commonly co-formulated Diclofenac. The method is simple, sensitive, precise and could efficiently determine the five components by a complementary application of two different techniques. The first is univariate second derivative method that was successfully applied for determination of Vitamin B12. The second is Multivariate Curve Resolution using the Alternating Least Squares method (MCR-ALS) by which an efficient resolution and quantitation of the quaternary spectrally overlapped Vitamin B1, Vitamin B6, Benfotiamine and Diclofenac sodium were achieved. The effect of different constraints was studied and the correlation between the true spectra and the estimated spectral profiles were found to be 0.9998, 0.9983, 0.9993 and 0.9933 for B1, B6, Benfotiamine and Diclofenac, respectively. All components were successfully determined in tablets and capsules and the results were compared to HPLC methods and they were found to be statistically non-significant.
开发了一种新方法，用于光谱分辨率分析以及进一步测定包含复合维生素B（B1、B6、B12和苯磷硫胺）以及常用的共同配方双氯芬酸的五组分混合物。该方法简单、灵敏、精确，并且可以通过两种不同技术的互补应用有效地测定这五种组分。第一种是单变量二阶导数法，已成功应用于维生素B12的测定。第二种是使用交替最小二乘法的多元曲线分辨法（MCR-ALS），通过该方法实现了对光谱重叠的维生素B1、维生素B6、苯磷硫胺和双氯芬酸钠的四元高效分辨和定量。研究了不同约束条件的影响，发现B1、B6、苯磷硫胺和双氯芬酸的真实光谱与估计光谱轮廓之间的相关性分别为0.9998、0.9983、0.9993和0.9933。所有组分均在片剂和胶囊中成功测定，结果与高效液相色谱法进行了比较，发现无统计学显著差异。
