Morelli B
Universita' degli Studi, Bari, Dipartimento di Chimica, Italy.
J Pharm Sci. 1995 Jan;84(1):34-7. doi: 10.1002/jps.2600840109.
A new method for determining a quaternary mixture of vitamins B6, B1, and B12 and uridine 5'-triphosphate (UPT) using second-derivative spectrophotometry is described. Calibration graphs were linear up to 30 micrograms/mL of vitamin B6 at 307 nm (r = 0.9999) and vitamin B1 at 282.7 nm (r = 0.9997) and up to 35 micrograms/mL of vitamin B12 at 360.5-374 nm, peak-to-peak (r = 0.9999), and UTP at 261 nm (r = 1.0000). Detection limits at the p = 0.05 level of significance were calculated to be 0.20, 0.46, 0.22, and 0.20 microgram/mL for vitamins B6, B1, and B12 and UTP, respectively. An exhaustive statistical analysis of the experimental data was performed to confirm the validity of the method. The procedure does not require any separation step or solving of equations. The method was successfully applied for determining synthetic mixtures and commercial injections for these drugs.
本文描述了一种使用二阶导数分光光度法测定维生素B6、B1、B12和尿苷5'-三磷酸（UTP）四元混合物的新方法。校准曲线在307 nm处维生素B6浓度高达30微克/毫升（r = 0.9999）、282.7 nm处维生素B1浓度高达30微克/毫升（r = 0.9997）、360.5 - 374 nm处维生素B12峰峰值浓度高达35微克/毫升（r = 0.9999）以及261 nm处UTP浓度高达35微克/毫升（r = 1.0000）时呈线性。在显著性水平p = 0.05时，计算得出维生素B6、B1、B12和UTP的检测限分别为0.20、0.46、0.22和0.20微克/毫升。对实验数据进行了详尽的统计分析以确认该方法的有效性。该方法无需任何分离步骤或求解方程。该方法已成功应用于测定这些药物的合成混合物和商业注射液。
