Li Haihong, Chen Lu, Zeng Shaopeng, Li Xuexue, Zhang Xiang, Lin Changmin, Zhang Mingjun, Xie Sitian, He Yunpu, Shu Shenyou, Yang Lvjun, Tang Shijie, Fu Xiaobing
Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, PR China; Research Center for Translational Medicine, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, PR China.
Burn and Plastic Surgery, The Second Affiliated Hospital, Shantou University Medical College, North Dongxia Road, Shantou, Guangdong Province 515041, PR China.
Exp Cell Res. 2015 Mar 1;332(1):67-77. doi: 10.1016/j.yexcr.2015.01.014. Epub 2015 Jan 30.
Severe burn results in irreversible damage to eccrine sweat glands, for which no effective treatment is available. Interaction between the extracellular matrix and epithelial cells is critical for proper three-dimensional organization and function of the epithelium.
Matrigel-embedded eccrine sweat gland cells were subcutaneously implanted into the inguinal regions of nude mice. Two weeks later, the Matrigel plugs were removed and evaluated for series of detection items.
Sweat gland cells developed into sweat gland-like structures in the Matrigel plugs based on: (1) de novo formation of tubular-like structures with one or more hollow lumens, (2) expression of epithelial and sweat gland markers (pancytokeratin, CK5/7/14/19, α-SMA and CEA), (3) basement membrane formation, (4) myoepithelial cells presenting in and encompassing the tubular-like structures, (5) cellular polarization, evident by the expression of tight junction proteins (claudin-1 and ZO-2), anchoring junctions (desmoglein-1 and -2 and E-cadherin) and CEA in the luminal membrane, (6) expression of proteins related to sweat secretion and absorption (Na(+)-K(+)-ATPase α/β, Na(+)-K(+)-2Cl-cotranspoter 1, Na(+)/H(+) exchanger 1, aquaporin-5, epithelial sodium channel, cystic fibrosis transmembrane conductance regulator, potassium channel and vacuolar-type H+-ATPase), and (7) about 20% of the tubular-like structures are de novo coils and 80% are de novo ducts.
This study provides not only an excellent model to study eccrine sweat gland development, cytodifferentiation and reconstitution, but also an in vivo model for regeneration of eccrine sweat glands.
严重烧伤会导致外分泌汗腺发生不可逆损伤，目前尚无有效的治疗方法。细胞外基质与上皮细胞之间的相互作用对于上皮组织正常的三维结构和功能至关重要。
将包埋于基质胶中的外分泌汗腺细胞皮下植入裸鼠腹股沟区域。两周后，取出基质胶栓并进行一系列检测项目评估。
汗腺细胞在基质胶栓中发育成汗腺样结构，依据如下：（1）形成具有一个或多个中空管腔的管状样结构；（2）上皮和汗腺标志物（全细胞角蛋白、CK5/7/14/19、α平滑肌肌动蛋白和癌胚抗原）的表达；（3）基底膜形成；（4）肌上皮细胞存在于管状样结构内并围绕该结构；（5）细胞极化，表现为紧密连接蛋白（闭合蛋白-1和ZO-2）、锚定连接蛋白（桥粒芯糖蛋白-1和-2以及E-钙黏蛋白）和管腔膜中癌胚抗原的表达；（6）与汗液分泌和吸收相关的蛋白（钠钾ATP酶α/β、钠钾氯共转运体1、钠氢交换体1、水通道蛋白-5、上皮钠通道、囊性纤维化跨膜传导调节因子、钾通道和液泡型氢离子ATP酶）的表达；（7）约20%的管状样结构为新生螺旋管，80%为新生导管。
本研究不仅提供了一个研究外分泌汗腺发育、细胞分化和重建的优秀模型，还提供了一个外分泌汗腺再生的体内模型。
