Cop N, Uyttebroek A P, Sabato V, Bridts C H, De Clerck L S, Ebo D G
Department of Immunology, Allergology, Rheumatology, Faculty of Medicine and Health Science, University of Antwerp, Antwerp University Hospital, Antwerpen, 2610, Belgium.
Cytometry B Clin Cytom. 2016 May;90(3):285-8. doi: 10.1002/cyto.b.21226. Epub 2015 Apr 27.
Histamine and its release can be studied by multicolor flow cytometry on a single cell level by an enzyme affinity method (HistaFlow®). However, for the time-being, the clinical and scientific application of the HistaFlow® technique remains limited. This study aims at verifying the reliability of the HistaFlow® as an instrument to quantify IgE-mediated basophil responses to drugs, i.e., rocuronium, which are believed to be less potent basophil activators than large proteinaceous allergens. Ten patients and three exposed control individuals were included in this study. Each subject underwent in vitro basophil activation tests (HistaFlow®) with 0.16 and 1.6 mmol/L rocuronium. Patients showed an activation of basophils ranging from 11 to 86% of CD63 positive basophils and a median histamine release per cell from 68 to 100% after stimulation with an optimal concentration of 1.6 mmol/L rocuronium. For the control individuals no activation was demonstrable. This study confirms that the HistaFlow® technique is a reliable tool to study histamine release by individual cells in response to drugs. Although the HistaFlow® technique will probably not add to the diagnostic management of rocuronium allergy, our findings suggest that the technique could constitute an important asset for future studies on the pathomechanism(s) of immediate drug hypersensitivity reactions. © 2015 International Clinical Cytometry Society.
组胺及其释放情况可通过酶亲和法（HistaFlow®）在单细胞水平上采用多色流式细胞术进行研究。然而，目前HistaFlow®技术的临床和科学应用仍然有限。本研究旨在验证HistaFlow®作为一种仪器用于量化IgE介导的嗜碱性粒细胞对药物（即罗库溴铵）的反应的可靠性，据信罗库溴铵作为嗜碱性粒细胞激活剂的效力低于大型蛋白质过敏原。本研究纳入了10名患者和3名暴露对照个体。每位受试者接受了0.16和1.6 mmol/L罗库溴铵的体外嗜碱性粒细胞激活试验（HistaFlow®）。在用1.6 mmol/L最佳浓度的罗库溴铵刺激后，患者的嗜碱性粒细胞激活率在CD63阳性嗜碱性粒细胞的11%至86%之间，每个细胞的组胺释放中位数在68%至100%之间。对于对照个体，未显示出激活情况。本研究证实，HistaFlow®技术是研究单个细胞对药物反应时组胺释放的可靠工具。尽管HistaFlow®技术可能不会为罗库溴铵过敏的诊断管理增添内容，但我们的研究结果表明，该技术可能成为未来关于即刻药物超敏反应发病机制研究的一项重要资产。© 2015国际临床细胞计量学会。
