Krausz Kelsey L, Bose Jeffrey L
Department of Microbiology, Molecular Genetics, and Immunology, The University of Kansas Medical Center, MSN 3029, 3901 Rainbow Boulevard, Kansas City, KS, 66160, USA.
Methods Mol Biol. 2016;1373:63-8. doi: 10.1007/7651_2014_185.
The ability to move DNA between Staphylococcus strains is essential for the genetic manipulation of this bacterium. Often in the Staphylococci, this is accomplished through transduction using generalized transducing phage and can be performed in different ways and therefore the presence of two transduction procedures in this book. The following protocol is a relatively easy-to-perform, broth-based procedure that we have used extensively to move both plasmids and chromosomal fragments between strains of Staphylococcus aureus.
在葡萄球菌菌株之间转移DNA的能力对于该细菌的基因操作至关重要。在葡萄球菌中，这通常通过使用广义转导噬菌体进行转导来实现，并且可以以不同方式进行，因此本书中有两种转导程序。以下方案是一种相对易于操作的基于肉汤的程序，我们已广泛使用该程序在金黄色葡萄球菌菌株之间转移质粒和染色体片段。
