Sieber-Blum M
Department of Anatomy and Cellular Biology, Medical College of Wisconsin, Milwaukee 53226.
Science. 1989 Mar 24;243(4898):1608-11. doi: 10.1126/science.2564699.
Clonal cultures and monoclonal antibodies against a lineage-specific epitope, stage-specific embryonic antigen-1 (SSEA-1) were used to analyze the commitment of quail neural crest cells to the sensory neuron pathway. There were two distinct populations of sensory cells at the time of gangliogenesis. Postmitotic neuroblasts that remained in close association with the neural tube coexisted with a large number of pluripotent cells that formed the leading edge of the emigrating cells and gave rise to sensory and autonomic neuroblasts and to melanocytes. The data suggest a dual origin of spinal sensory neuroblasts and a predominantly late divergence of the autonomic and sensory lineages.
利用针对谱系特异性表位——阶段特异性胚胎抗原-1（SSEA-1）的克隆培养物和单克隆抗体，分析鹌鹑神经嵴细胞向感觉神经元途径的定向分化。在神经节形成时，存在两种不同类型的感觉细胞群体。与神经管紧密相连的有丝分裂后神经母细胞，与大量多能细胞共存，这些多能细胞形成了迁出细胞的前沿，并产生感觉和自主神经母细胞以及黑素细胞。数据表明脊髓感觉神经母细胞有双重起源，自主神经和感觉谱系主要在后期发生分化。
