Departamento de Ingeniería Química, CUCEI-Universidad de Guadalajara, Blvd. M. García Barragán 1451, C.P. 44430, Guadalajara, Jalisco, Mexico.
División de Ciencias Ambientales. Instituto Potosino de Investigación Científica y Tecnológica A.C., Camino a la Presa San José No. 2055, Col. Lomas 4(a) Sección, C.P. 78216, San Luis Potosí, SLP, Mexico.
Bioresour Technol. 2015 Apr;181:191-9. doi: 10.1016/j.biortech.2015.01.036. Epub 2015 Jan 17.
Evaluation of diluted acid hydrolysis for sugar extraction from cooked and uncooked Agave tequilana bagasse and feasibility of using the hydrolysates as substrate for methane production, with and without nutrient addition, in anaerobic sequencing batch reactors (AnSBR) were studied. Results showed that the hydrolysis over the cooked bagasse was more effective for sugar extraction at the studied conditions. Total sugars concentration in the cooked and uncooked bagasse hydrolysates were 27.9 g/L and 18.7 g/L, respectively. However, 5-hydroxymethylfurfural was detected in the cooked bagasse hydrolysate, and therefore, the uncooked bagasse hydrolysate was selected as substrate for methane production. Interestingly, results showed that the AnSBR operated without nutrient addition obtained a constant methane production (0.26 L CH4/g COD), whereas the AnSBR operated with nutrient addition presented a gradual methane suppression. Molecular analyses suggested that methane suppression in the experiment with nutrient addition was due to a negative effect over the archaeal/bacterial ratio.
研究了稀释酸水解法从蒸煮和未蒸煮的龙舌兰 tequilana 蔗渣中提取糖的效果，并评估了在添加和不添加营养物的条件下，利用水解物作为甲烷生产的基质在厌氧序批式反应器 (AnSBR) 中的可行性。结果表明，在研究条件下，蒸煮蔗渣的水解对糖提取更为有效。蒸煮和未蒸煮蔗渣水解物中的总糖浓度分别为 27.9 g/L 和 18.7 g/L。然而，在蒸煮蔗渣水解物中检测到了 5-羟甲基糠醛，因此，选择未蒸煮的蔗渣水解物作为甲烷生产的基质。有趣的是，结果表明，在不添加营养物的 AnSBR 中可以获得恒定的甲烷产量（0.26 L CH4/g COD），而在添加营养物的 AnSBR 中则呈现出逐渐的甲烷抑制。分子分析表明，在添加营养物的实验中，甲烷抑制是由于对古菌/细菌比例的负面影响。
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