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对从伊朗西北部大不里士当地市场采集的肉鸡胴体中恩诺沙星和氯霉素残留的调查。

Investigation of enrofloxacin and chloramphenicol residues in broiler chickens carcasses collected from local markets of tabriz, northwestern iran.

作者信息

Ebrahimzadeh Attari Vahideh, Mesgari Abbasi Mehran, Abedimanesh Nasim, Ostadrahimi Alireza, Gorbani Abolfazl

机构信息

Department of Nutrition & Biochemistry, Tabriz University of Medical Sciences, Tabriz, Iran.

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.

出版信息

Health Promot Perspect. 2014 Dec 30;4(2):151-7. doi: 10.5681/hpp.2014.020. eCollection 2014.

Abstract

BACKGROUND

The present study was aimed to determine the residual amounts of chloramphenicol and enrofloxacin in broiler chickens muscle and liver sam-ples gathered from local markets of Tabriz City, northwestern Iran.

METHODS

Ninety broiler chickens carcasses were collected from different local markets of Tabriz, during July/August 2013. Random samples of thigh and breast muscle and liver were gathered and kept at -80(°)C until analyzes. The samples were then assayed using enzyme-linked immunosorbent assay (ELISA) according to the protocol of each antibiotic kit. Data were statistically analyzed using the computer program SAS 9.1.

RESULTS

Eighty two samples (91/1 %) contained residues of enrofloxacin, alt-hough mean (±SD)of enrofloxacin concentration was lower than the European Union maximum residue limits(MRLs) value (P<0.001). Moreover, 28 (31/1 %) had detectable concentrations of chloramphenicol while it was not defined any MRLs value for chloramphenicol because its using has been forbidden in food animals.

CONCLUSION

The frequency of contamination with enrofloxacin was considerable for the analyzed samples. Furthermore, the existence of chloramphenicol in almost one third of samples seems to be a public health threat due to its illegal use in food animals including poultry.

摘要

背景

本研究旨在测定从伊朗西北部大不里士市当地市场采集的肉鸡肌肉和肝脏样本中氯霉素和恩诺沙星的残留量。

方法

2013年7月/8月期间,从大不里士市不同的当地市场收集了90只肉鸡尸体。随机采集大腿、胸肌和肝脏样本,并保存在-80℃直至分析。然后根据每个抗生素试剂盒的方案,使用酶联免疫吸附测定法(ELISA)对样本进行检测。使用计算机程序SAS 9.1对数据进行统计分析。

结果

82个样本(91.1%)含有恩诺沙星残留,尽管恩诺沙星浓度的平均值(±标准差)低于欧盟最大残留限量(MRLs)值(P<0.001)。此外,28个样本(31.1%)检测到氯霉素浓度,而由于其在食用动物中已被禁止使用,因此未对氯霉素定义任何MRLs值。

结论

在所分析的样本中,恩诺沙星污染的频率相当高。此外,由于氯霉素在包括家禽在内的食用动物中非法使用,近三分之一的样本中存在氯霉素似乎对公共卫生构成威胁。

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