Laboratoire d'Ecologie et Environnement-Unité Associée au CNRST, URAC 32, Unité Associée au CNERS, Département de Biologie, Faculté des Sciences Semlalia, Université Cadi Ayyad, BP 2390, Marrakech, Maroc.
Ecotoxicol Environ Saf. 2012 Feb;76(2):187-92. doi: 10.1016/j.ecoenv.2011.10.012. Epub 2011 Nov 1.
The genotoxicity of quinolone and fluroquinolones was assessed using the micronucleus (MN) test on Vicia faba roots by direct contact exposure to a solid matrix. Plants were exposed to quinolones (nalidixic acid) and fluoroquinolones (ciprofloxacin and enrofloxacin) alone or mixed with artificially contaminated soils. Four different concentrations of each of these antibiotics were tested (0.01, 0.1, 1 and 10 mg/Kg) for nalidixic acid and (0.005, 0.05, 0.5 and 5 mg/Kg) for ciprofloxacin and enrofloxacin. These antibiotics were also used in mixture. Exposure of Vicia faba plants to each antibiotic at the highest two concentrations showed significant MN induction. The lowest two concentrations had no significant genotoxic effect. The mixture of the three compounds induced a significant MN induction whatever the mixture tested, from 0.02 to 20 mg/Kg. The results indicated that a similar genotoxic effect was obtained with the mixture at 0.2 mg/Kg in comparison with each molecule alone at 5-10 mg/Kg. Data revealed a clear synergism of these molecules on Vicia faba genotoxicity.
采用蚕豆根尖微核直接接触法，评估了喹诺酮类和氟喹诺酮类药物的遗传毒性。将植物直接暴露于固体基质中，用喹诺酮（萘啶酸）和氟喹诺酮（环丙沙星和恩诺沙星）单独或与人工污染土壤混合进行暴露。对这些抗生素的每一种都测试了四个不同的浓度（萘啶酸为 0.01、0.1、1 和 10 mg/Kg，环丙沙星和恩诺沙星为 0.005、0.05、0.5 和 5 mg/Kg）。这些抗生素也以混合物的形式使用。在最高的两种浓度下，暴露于每种抗生素的蚕豆植物均显示出明显的微核诱导。最低的两种浓度则没有明显的遗传毒性作用。无论测试的混合物如何，这三种化合物的混合物均能诱导明显的微核诱导，从 0.02 到 20 mg/Kg。结果表明，在 0.2 mg/Kg 时，混合物的遗传毒性作用与单独使用每种分子在 5-10 mg/Kg 时相似。数据表明，这些分子对蚕豆的遗传毒性具有明显的协同作用。
