Comparative HPTLC fingerprinting profile and GC-MS analysis of raw and purified () by purification method.

BACKGROUND

is an oleo gum resin obtained from the plant (Arn.) Bhand., used in medicines for various ailments like anti-inflammatory conditions, hyperlipidemia, thyroid disorders etc. Guggulsterones E & Z are responsible for these broad ranges of pharmacological actions. It is recommended to do (purification) before incorporating it into medicinal formulations. , an text, emphasizes the purification of Guggulu in a particular media, which is a long run practice in Kerala.

OBJECTIVES

To compare the physicochemical and phytochemical parameters, quantitative estimation of Guggulsterone E & Z using high performance thin layer chromatography (HPTLC) and qualitative gas chromatography-mass spectrometry (GC-MS) analysis of Guggulu before and after purification.

METHODS

of was performed in Water boiled with crushed fresh leaves of Neem () and fresh rhizome of turmeric () using a special equipment (). Preliminary physicochemical and phytochemical evaluation, quantification of Guggulsterones E & Z using HPTLC and GC-MS analysis of raw and purified were performed.

RESULTS

Phytochemical evaluation of metabolites revealed marked variations. The mean concentrations of Guggulsterone E & Z showed significant differences before and after purification (p<0.01). On GC-MS analysis, it was found that few new compounds were added in the purified .

CONCLUSIONS

Therapeutic efficacy of might have enhanced after traditional purification.