Dersch Julie Maria, Nguyen Tam T T N, Østergaard Jesper, Stürup Stefan, Gammelgaard Bente
Department of Pharmacy, Faculty of Health and Medical Sciences, University of Copenhagen, Universitetsparken 2, 2100, Copenhagen, Denmark.
Anal Bioanal Chem. 2015 Apr;407(10):2829-36. doi: 10.1007/s00216-015-8507-7. Epub 2015 Feb 4.
Human serum albumin (HSA) is the most abundant protein in the human plasma. HSA has several physiological roles in the human body, including storage and transport. Owing to the predominance of albumin in plasma, HSA is often involved in the protein binding of drugs. The aim of this work was to develop a selective, quantitative method for determining albumin in plasma with the purpose of clarifying the fate of metal-based drugs in biological systems. The method can also be applied for determination of urine albumin, which is of relevance in diagnostics of kidney disease. A selective method for quantification of HSA based on labelling the protein with iophenoxic acid (IPA) was developed. Samples were subjected to size exclusion chromatography (SEC) and detection by inductively coupled plasma mass spectrometry (ICP-MS) monitoring iodine and platinum. The iodine signal for the HSA-IPA complex showed linearity in the range 1 to 250 mg L(-1). The precision was 3.7% and the accuracy 100.7% determined by analysis of a certified HSA reference material. The limit of detection (LOD) and limit of quantification (LOQ) were 0.23 and 9.79 mg L(-1), respectively. The method was applied for analysis of HSA in human plasma and urine samples and for studying the binding of cisplatin to proteins in the human plasma.
人血清白蛋白（HSA）是人体血浆中含量最丰富的蛋白质。HSA在人体中具有多种生理作用，包括储存和运输。由于白蛋白在血浆中占主导地位，HSA常参与药物的蛋白结合。这项工作的目的是开发一种选择性、定量测定血浆中白蛋白的方法，以阐明金属基药物在生物系统中的命运。该方法也可用于测定尿白蛋白，这在肾脏疾病诊断中具有相关性。开发了一种基于用碘苯氧乙酸（IPA）标记蛋白质来定量HSA的选择性方法。样品经过尺寸排阻色谱（SEC）分离，并用电感耦合等离子体质谱（ICP-MS）监测碘和铂进行检测。HSA-IPA复合物的碘信号在1至250 mg L⁻¹范围内呈线性。通过分析一种经认证的HSA参考物质，精密度为3.7%，准确度为100.7%。检测限（LOD）和定量限（LOQ）分别为0.23和9.79 mg L⁻¹。该方法用于分析人血浆和尿液样本中的HSA，并用于研究顺铂与人血浆中蛋白质的结合。
