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鸡经鼻内接种多杀性巴氏杆菌A:1血清型重组外膜蛋白H免疫后的保护性

Protectivity conferred by immunization with intranasal recombinant outer membrane protein H from Pasteurella multocida serovar A:1 in chickens.

作者信息

Thanasarasakulpong Arunee, Poolperm Pichayanut, Tankaew Pallop, Sawada Takuo, Sthitmatee Nattawooti

机构信息

Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai 50100, Thailand.

出版信息

J Vet Med Sci. 2015 Mar;77(3):321-6. doi: 10.1292/jvms.14-0532. Epub 2014 Dec 20.

DOI:10.1292/jvms.14-0532
PMID:25650149
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4383778/
Abstract

Recombinant outer membrane protein H (rOmpH) is a potential fowl cholera vaccine candidate. The present study was aimed at developing rOmpH formulations for intranasal administration. The rOmpH was purified and formulated with either Escherichia coli enterotoxin B (LTB) or CpG oligodeoxynucleotides (ODN) as an adjuvant. Antibody responses in chickens intranasally immunized with rOmpH in combination with 2 different adjuvants were significantly increased (P<0.05) post immunization. Chicken survival rates showed that rOmpH formulated with ODN and LTB elicited 90% and 70% protection, respectively. Our findings indicated that rOmpH formulated with ODN elicited protection better than that formulated with LTB. Therefore, the vaccines formulations in the present study can be considered new intranasal vaccine formulations for fowl cholera in chickens.

摘要

重组外膜蛋白H（rOmpH）是一种潜在的禽霍乱疫苗候选物。本研究旨在开发用于鼻内给药的rOmpH制剂。rOmpH被纯化，并与大肠杆菌肠毒素B（LTB）或CpG寡脱氧核苷酸（ODN）作为佐剂进行配制。用rOmpH联合2种不同佐剂进行鼻内免疫的鸡在免疫后抗体反应显著增加（P<0.05）。鸡的存活率表明，用ODN和LTB配制的rOmpH分别引起90%和70%的保护作用。我们的研究结果表明，用ODN配制的rOmpH比用LTB配制的rOmpH诱导的保护效果更好。因此，本研究中的疫苗制剂可被认为是鸡禽霍乱新的鼻内疫苗制剂。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b4c/4383778/34945d7cb853/jvms-77-321-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b4c/4383778/823b9cd25463/jvms-77-321-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b4c/4383778/ffb41a28ca71/jvms-77-321-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b4c/4383778/34945d7cb853/jvms-77-321-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b4c/4383778/823b9cd25463/jvms-77-321-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b4c/4383778/ffb41a28ca71/jvms-77-321-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2b4c/4383778/34945d7cb853/jvms-77-321-g003.jpg

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