de Jonge N, Schommer G, Krijger F W, Feldmeier H, Zwingenberger K, Steiner A, Bienzle U, Deelder A M
Laboratory of Parasitology, Medical Faculty, State University of Leiden, The Netherlands.
Acta Trop. 1989 Mar;46(2):115-20. doi: 10.1016/0001-706x(89)90005-3.
The presence of the schistosome circulating anodic antigen (CAA) in serum of Schistosoma intercalatum-infected patients from Gabon has been investigated using an enzyme-linked immunosorbent assay (ELISA). Blood samples were collected from 10 endemic controls, 29 patients which excreted viable S. intercalatum eggs in rectal mucosa and stool, six persons in which only non-viable eggs were found in rectal biopsy specimens and one person in which besides non-viable eggs a small number of viable eggs was found in the rectal biopsy specimen. CAA, a genus-specific antigen, could be demonstrated in 58.6% of the patients with S. intercalatum eggs in their stools. In comparison to S. mansoni infections, very light infections (0.6 eggs per gram faeces) could be detected by the ELISA. A strong correlation between parasite burden (eggs per gram faeces) and antigen-level (CAA-titer) was found (Spearman's rho = 0.65). Only one positive ELISA-results was found in the group with solely non-viable eggs in rectal tissue. As no false positive results were detected for the negative controls, the present results suggest, in accordance with results earlier obtained for schistosomiasis mansoni, that only in active S. intercalatum infections is antigen demonstrable.
利用酶联免疫吸附测定法（ELISA）对加蓬感染间插血吸虫患者血清中的血吸虫循环阳极抗原（CAA）进行了检测。从10名地方性对照者、29名直肠黏膜和粪便中排出活的间插血吸虫卵的患者、6名直肠活检标本中仅发现非活卵的人和1名直肠活检标本中除非活卵外还发现少量活卵的人采集了血样。CAA是一种属特异性抗原，在58.6%粪便中有间插血吸虫卵的患者中可检测到。与曼氏血吸虫感染相比，ELISA可检测到非常轻度的感染（每克粪便0.6个卵）。发现寄生虫负荷（每克粪便中的卵数）与抗原水平（CAA滴度）之间存在强相关性（Spearman等级相关系数ρ = 0.65）。在直肠组织中仅含非活卵的组中仅发现1例ELISA阳性结果。由于未检测到阴性对照的假阳性结果，目前的结果表明，与早期获得的曼氏血吸虫病结果一致，仅在活动性间插血吸虫感染中可检测到抗原。
