Bicknell R J, Luckman S M, Inenaga K, Mason W T, Hatton G I
Department of Neuroendocrinology, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK.
Brain Res Bull. 1989 Feb;22(2):379-88. doi: 10.1016/0361-9230(89)90065-8.
Explants of adult rat neurohypophysis were maintained in culture for 14 days. The majority of cells present in the outgrowth of such cultures were identified as pituicytes on the basis of immunostaining for glial fibrillary acidic protein. Pituicytes were also stained by antisera to the membrane glycoprotein antigen Thy-1 and the extracellular matrix glycoprotein fibronectin. The cultures contained naloxone sensitive binding sites for the opioid receptor ligand [3H] dynorphin A 1-8 and peripheral-type benzodiazepine binding sites. Dynorphin binding was visualised over pituicytes following autoradiography. The morphology of cultured pituicytes was regulated by beta-adrenergic receptors present on the cells which, when activated, stimulated rapid transformation from a flattened irregular morphology to a stellate, process-bearing morphology. Dynorphin was without effect on the morphology of cultured pituicytes. These findings are discussed in the context of the known morphological plasticity of pituicytes in vivo.
成年大鼠神经垂体的外植体在培养中维持14天。基于对胶质纤维酸性蛋白的免疫染色，此类培养物生长物中存在的大多数细胞被鉴定为垂体细胞。垂体细胞也被抗膜糖蛋白抗原Thy-1和细胞外基质糖蛋白纤连蛋白的抗血清染色。培养物中含有对阿片受体配体[3H]强啡肽A 1-8的纳洛酮敏感结合位点和外周型苯二氮䓬结合位点。放射自显影后，在垂体细胞上可见强啡肽结合。培养的垂体细胞的形态受细胞上存在的β-肾上腺素能受体调节，当该受体被激活时，会刺激垂体细胞从扁平不规则形态迅速转变为有突起的星状形态。强啡肽对培养的垂体细胞的形态没有影响。这些发现是在已知垂体细胞在体内的形态可塑性的背景下进行讨论的。
