Yio M H N, Mac M J, Wong H S, Buenfeld N R
Concrete Durability Group, Department of Civil and Environmental Engineering, Imperial College London, UK.
J Microsc. 2015 May;258(2):151-69. doi: 10.1111/jmi.12228. Epub 2015 Feb 4.
In this paper, we present a new method to reconstruct large volumes of nontransparent porous materials at submicron resolution. The proposed method combines fluorescence laser scanning confocal microscopy with serial sectioning to produce a series of overlapping confocal z-stacks, which are then aligned and stitched based on phase correlation. The method can be extended in the XY plane to further increase the overall image volume. Resolution of the reconstructed image volume does not degrade with increase in sample size. We have used the method to image cementitious materials, hardened cement paste and concrete and the results obtained show that the method is reliable. Possible applications of the method such as three-dimensional characterization of the pores and microcracks in hardened concrete, three-dimensional particle shape characterization of cementitious materials and three-dimensional characterization of other porous materials such as rocks and bioceramics are discussed.
在本文中，我们提出了一种在亚微米分辨率下重建大量不透明多孔材料的新方法。该方法将荧光激光扫描共聚焦显微镜与连续切片相结合，以生成一系列重叠的共聚焦z轴堆叠图像，然后基于相位相关性对这些图像进行对齐和拼接。该方法可在XY平面上扩展，以进一步增加整体图像体积。重建图像体积的分辨率不会随着样品尺寸的增加而降低。我们已使用该方法对胶凝材料、硬化水泥浆体和混凝土进行成像，所得结果表明该方法是可靠的。文中还讨论了该方法的可能应用，如硬化混凝土中孔隙和微裂纹的三维表征、胶凝材料的三维颗粒形状表征以及岩石和生物陶瓷等其他多孔材料的三维表征。
