Mishra Ankita, Jain Anuja, Arora Naveen
Allergy and Immunology Laboratory, CSIR, Institute of Genomics and Integrative Biology (IGIB), Delhi 110007, India.
J Sci Food Agric. 2016 Jan 30;96(2):539-47. doi: 10.1002/jsfa.7121. Epub 2015 Mar 19.
Epitope identification provides valuable information essential for understanding antigen components involved in food allergic reactions. In the present study, an in silico approach is employed to map IgE binding epitopes of major and minor peanut allergens.
B-cell epitopes were identified for peanut (Arachis hypogaea) allergens, namely Ara h 5, 6, 7, 8, 9, 10 and 11. A total of 10 web servers were used in the study and 26 linear and 18 conformational epitopes were predicted by a combination of methods. The majority of the predicted B-cell residues were present in the coil regions and the highest percentage of hydrophilic residues were observed for Ara h 6 (70.49%). The absolute solvent accessibility for all the B-cell epitopes was >70%, indicating antibody recognition. The property distance index assessed for the predicted epitopes using SDAP showed that six linear epitopes shared similarity with soybean, hazelnut, tomato, maize, apple and banana allergens.
These findings suggest that the identified regions may share cross-reactivity with some of the known food allergens or may act as novel antigenic determinants. Further, B-cell epitopes of Ara h 1, 2 and 3 identified by in silico methods correlated well with the experimentally identified regions.
表位鉴定为理解食物过敏反应中涉及的抗原成分提供了至关重要的有价值信息。在本研究中，采用了一种计算机模拟方法来绘制主要和次要花生过敏原的IgE结合表位。
鉴定出了花生（落花生）过敏原的B细胞表位，即Ara h 5、6、7、8、9、10和11。本研究共使用了10个网络服务器，并通过多种方法组合预测出了26个线性表位和18个构象表位。预测的B细胞残基大多存在于卷曲区域，其中Ara h 6的亲水性残基百分比最高（70.49%）。所有B细胞表位的绝对溶剂可及性均>70%，表明可被抗体识别。使用SDAP对预测表位评估的特性距离指数表明，6个线性表位与大豆、榛子、番茄、玉米、苹果和香蕉过敏原具有相似性。
这些发现表明，所鉴定的区域可能与一些已知食物过敏原存在交叉反应，或者可能作为新的抗原决定簇。此外，通过计算机模拟方法鉴定出的Ara h 1、2和3的B细胞表位与实验鉴定的区域相关性良好。
