Morais Talita Cavalcante, Arruda Bruno Rodrigues, de Sousa Magalhães Hebert, Trevisan Maria Teresa Salles, de Araújo Viana Daniel, Rao Vietla Satyanarayana, Santos Flavia Almeida
Department of Physiology and Pharmacology, Faculty of Medicine, INCT-IBISAB-Brazilian Semi-Arid Institute of Biomedicine, 60430-270, Fortaleza, Ceará, Brazil.
Naunyn Schmiedebergs Arch Pharmacol. 2015 May;388(5):531-8. doi: 10.1007/s00210-015-1095-4. Epub 2015 Feb 5.
Our previous study has shown that mangiferin (MGF), a glucosylxanthone from Mangifera indica, exerts gastrointestinal prokinetic action involving a cholinergic mechanism. Postoperative ileus (POI) is a temporary disturbance in gastrointestinal motility following surgery, and intestinal inflammatory response plays a critical role in the pathogenesis of POI. The present study investigated to know whether MGF having anti-inflammatory and prokinetic actions can ameliorate the intestinal inflammation and impaired gastrointestinal transit seen in the mouse model of POI. Experimental POI was induced in adult male Swiss mice by standardized small intestinal manipulation (IM). Twenty-four hours later, gastrointestinal transit was assessed by charcoal transport. MGF was administered orally 1 h before the measurement of GIT. To evaluate the inflammatory response, plasma levels of proinflammatory cytokines TNF-α, IL-1β, IL-6, and chemokine MCP-1, and the myeloperoxidase activity, nitrate/nitrite level, and histological changes of ileum were determined in mice treated or not with MGF. Experimental POI in mice was characterized by decreased gastrointestinal transit and marked intestinal and systemic inflammatory response. MGF treatment led to recovery of the delayed intestinal transit induced by IM. MGF in ileum significantly inhibited the myeloperoxidase activity, a marker of neutrophil infiltration, and nitrate/nitrite level and reduced the plasma levels of TNF-α, IL-1β, IL-6, and MCP-1 as well. MGF treatment ameliorates the intestinal inflammatory response and the impaired gastrointestinal motility in the mouse model of POI.
我们之前的研究表明，芒果苷（MGF），一种来自芒果的葡糖基氧杂蒽酮，具有涉及胆碱能机制的胃肠促动力作用。术后肠梗阻（POI）是手术后胃肠道蠕动的一种暂时紊乱，肠道炎症反应在POI的发病机制中起关键作用。本研究旨在探讨具有抗炎和促动力作用的MGF是否能改善POI小鼠模型中出现的肠道炎症和胃肠转运受损情况。通过标准化的小肠操作（IM）在成年雄性瑞士小鼠中诱导实验性POI。24小时后，通过炭末传输评估胃肠转运。在测量胃肠道之前1小时口服给予MGF。为了评估炎症反应，测定了用或未用MGF处理的小鼠血浆中促炎细胞因子TNF-α、IL-1β、IL-6和趋化因子MCP-1的水平，以及回肠的髓过氧化物酶活性、硝酸盐/亚硝酸盐水平和组织学变化。小鼠实验性POI的特征是胃肠转运减少以及明显的肠道和全身炎症反应。MGF治疗导致IM诱导的延迟肠道转运恢复。MGF在回肠中显著抑制髓过氧化物酶活性（中性粒细胞浸润的标志物）以及硝酸盐/亚硝酸盐水平，并降低血浆中TNF-α、IL-1β、IL-6和MCP-1的水平。MGF治疗改善了POI小鼠模型中的肠道炎症反应和胃肠动力受损情况。
