Butler W L
Ciba Found Symp. 1978(61):237-56. doi: 10.1002/9780470720431.ch13.
Tripartite and bipartite models for the photochemical apparatus of photosynthesis are presented and examined. It is shown that the equations for the yields of fluorescence from the different parts of the photochemical apparatus of the tripartite model transform into the simple equations of the bipartite formulation when the probability for energy transfer from the light-harvesting chlorophyll a/b complex to photosystem II is unity. The nature of the 695 and 735 nm fluorescence bands which appear in the emission spectrum of chloroplasts at low temperature is examined. It is proposed that these bands are due to fluorescence from energy-trapping centres which form in the antenna chlorophyll of photosystem II and photosystem I on cooling to low temperature. Even though these fluorescence emissions can be regarded as low temperature artifacts since they are not present at physiological temperatures, they nevertheless are proportional to the excitation energy in the two photosystems and can be used to monitor energy distribution in the photochemical apparatus. However, the question of their artifactual nature is crucial to the interpretation of fluorescence-lifetime measurements at low temperature.
本文提出并研究了光合作用光化学装置的三方模型和两方模型。结果表明，当从捕光叶绿素a/b复合体到光系统II的能量转移概率为1时，三方模型光化学装置不同部分的荧光产率方程可转化为两方模型的简单方程。研究了低温下叶绿体发射光谱中出现的695和735 nm荧光带的性质。有人提出，这些谱带是由于在冷却到低温时，光系统II和光系统I的天线叶绿素中形成的能量捕获中心发出的荧光。尽管这些荧光发射可被视为低温假象，因为它们在生理温度下不存在，但它们与两个光系统中的激发能成正比，可用于监测光化学装置中的能量分布。然而，它们的假象性质问题对于低温下荧光寿命测量的解释至关重要。
