Seely G R
Ciba Found Symp. 1978(61):41-59. doi: 10.1002/9780470720431.ch3.
There are several reasons for suspecting that there is a specific interaction between chlorophyll and galactolipids in the chloroplast. The model system described is intended to detect association of chlorophyll with polar lipids and other surfactants at a hydrocarbon-water interface. It consists of chlorophyll and other lipids or surfactants absorbed to the surface of polyethylene particles, which have been swelled with undecane to allow the lipophilic parts of these molecules to be anchored firmly in the hydrocarbon substrate. The absorption spectrum of adsorbed chlorophyll is usually modified by the presence of surfactant, and usually in the direction of decreased order of aggregation. Spectra in the presence of glycolipids in particular seem peculiar to the surfactant. The particles are strongly fluorescent, at room temperature as well as at 77K, and emission bands from aggregated chlorophyll species are observed along with fluorescence of monomeric chlorophyll.
怀疑叶绿体中叶绿素与半乳糖脂之间存在特定相互作用有几个原因。所描述的模型系统旨在检测叶绿素与极性脂质和其他表面活性剂在烃 - 水界面处的缔合。它由吸附在聚乙烯颗粒表面的叶绿素和其他脂质或表面活性剂组成，这些颗粒已用十一烷溶胀，以使这些分子的亲脂部分牢固地锚定在烃基质中。吸附叶绿素的吸收光谱通常会因表面活性剂的存在而改变，并且通常朝着聚集程度降低的方向变化。特别是在糖脂存在下的光谱似乎对表面活性剂而言很特殊。这些颗粒在室温以及77K时都有很强的荧光，并且除了单体叶绿素的荧光外，还观察到聚集叶绿素物种的发射带。
