Yang L
J Gen Physiol. 1966 Mar;49(4):763-80. doi: 10.1085/jgp.49.4.763.
Using Weber's method of "matrix analysis" for the estimation of the number of fluorescent species contributing to the emission of a sample, it is shown that the fluorescence(1) band in spinach chloroplast fragments at room temperature originates in two species of chlorophyll a. Emission spectra obtained upon excitation with different wavelengths of light (preferentially absorbed in chlorophyll a or b) are presented. Upon cooling to - 196 degrees C, the fluorescence efficiency increases about twentyfold. Two additional bands, that now appear at 696 and 735 mmicro, suggest the participation of four molecular species. Emission spectra observed at different concentrations of chloroplast fragments with excitation in chlorophyll a and b and excitation spectra for different concentrations of chloroplast fragments and measurements at 685 and 760 mmicro are presented. Two of the four emission bands may belong to pigment system I and two to system II. The 685, 696, and 738 mmicro bands respond differently to temperature changes. In the -196 degrees C to -150 degrees C range, the intensity of the 685 mmicro band remains constant, and that of the 696 mmicro band decreases twice as fast as that of the 738 mmicro band.
使用韦伯的“矩阵分析”方法来估算对样品发射有贡献的荧光物种数量，结果表明，室温下菠菜叶绿体片段中的荧光（1）带起源于两种叶绿素a。文中给出了用不同波长的光（优先被叶绿素a或b吸收）激发时获得的发射光谱。冷却至-196℃时，荧光效率增加约二十倍。另外出现的两条带，分别位于696和735微米处，表明有四种分子物种参与。文中还给出了在叶绿素a和b激发下不同浓度叶绿体片段的发射光谱，以及不同浓度叶绿体片段的激发光谱和在685和760微米处的测量结果。四条发射带中的两条可能属于色素系统I，另外两条属于系统II。685、696和738微米的带对温度变化的响应不同。在-196℃至-150℃范围内，685微米带的强度保持不变，696微米带强度下降的速度是738微米带的两倍。
