Rahimi Ebrahim, Kazemeini Hamid Reza, Salajegheh Mohammad
Department of Food Hygiene, College of Veterinary Medicine, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran;
Young Researchers Club, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran;
Vet Res Forum. 2012 Winter;3(1):15-7.
Enterohemorrhagic Escherichia coli (EHEC) of the O157:H7 serotype is a worldwide zoonotic pathogen responsible for the majority of severe cases of human EHEC disease. The aim of the present study was to investigate the prevalence of E. coli O157: H7/NM in raw meat samples from two provinces of Iran. During a period from March 2010 to March 2011. Two hundred and ninety five raw meat samples were collected from beef (n= 85), camel, (n= 50), sheep (n= 62), goat (n= 60), and water buffalo (n=38). Fourteen (4.7%) of the 295 samples were positive for E. coli O157. The highest prevalence of E. coli O157 was found in beef samples (8.2%), followed by water buffalo (5.3%), sheep (4.8%), camel (2.0%), and goat (1.7%). Of fourteen E. coli O157 isolates, only one was determined to be serotype O157: H7 while 13 were determined as serotype O157: NM. Of the 14 E. coli O157:H7/NM isolates, one, four, two, and one strains were positive for stx1, stx2, eaeA and ehlyA genes, respectively. The prevalence of this organism varied between seasons with the highest prevalence of E. coli O157 occurring in summer (9.3%). The results of this study showed that beef and water buffalo meat are a significant source for human EHEC E. coli O157:H7/NM infection in Iran. The data reported in this study provides some useful baseline in formation for future research such as molecular or epidemiologic works.
O157:H7血清型的肠出血性大肠杆菌（EHEC）是一种全球范围内的人畜共患病原体，是导致大多数人类严重EHEC疾病病例的原因。本研究的目的是调查伊朗两个省份生肉样本中大肠杆菌O157:H7/NM的流行情况。在2010年3月至2011年3月期间，从牛肉（n = 85）、骆驼（n = 50）、绵羊（n = 62）、山羊（n = 60）和水牛（n = 38）中收集了295份生肉样本。295份样本中有14份（4.7%）大肠杆菌O157呈阳性。大肠杆菌O157在牛肉样本中的流行率最高（8.2%），其次是水牛（5.3%）、绵羊（4.8%）、骆驼（2.0%）和山羊（1.7%）。在14株大肠杆菌O157分离株中，只有1株被确定为O157:H7血清型，而13株被确定为O157:NM血清型。在14株大肠杆菌O157:H7/NM分离株中，分别有1株、4株、2株和1株stx1、stx2、eaeA和ehlyA基因呈阳性。该生物体的流行率随季节变化，大肠杆菌O157的流行率在夏季最高（9.3%）。本研究结果表明，牛肉和水牛肉是伊朗人类感染EHEC大肠杆菌O157:H7/NM的重要来源。本研究报告的数据为未来的研究（如分子或流行病学研究）提供了一些有用的基线信息。
