Hajibemani Abolfazl, Sharifiyazdi Hassan, Mirzaei Abdolah, Rowshan Ghasrodashti Abbas
PhD Student at Department of Clinical Sciences, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran;
Department of Clinical Sciences, Faculty of Veterinary Medicine, Shiraz University, Shiraz, Iran;
Vet Res Forum. 2012 Winter;3(1):37-43.
Uterine infection is one of the reproductive diseases that can have disturbing postpartum uterine health in cattle. Therefore, identification of resistant genotypes to uterine infection is important. Lactoferrin (LF) is one of the major antimicrobial compounds in the normal uterine discharges of cows. We hypothesized that allelic diversity in LF gene may contribute to susceptibility or resistance to uterine infection. We investigated the single nucleotide polymorphism genes identified in the 5' untranslated region (5'-UTR, position +32) of the LF gene using Allele-specific PCR method in cows with and without uterine infection. Blood samples were collected from 89 multiparous Holstein dairy cows with a history of uterine infection (n = 51), and cows without disease as the control group (n= 38). The results indicated the presence of different proportion of polymorphisms (G > C) in the 5'-UTR area of cows in the all groups. The results of Allele specific PCR was in complete agreement with sequencing method. Statistical analysis did not show any statistically significant correlation between disease and SNP in 5'-UTR. While, there was a significant difference in the mean of reproductive parameters of cows without polymorphism compare to those of with SNP in 5'-UTR. Cows with +32:CC genotype and +32:GC genotype (cows with SNP in UTR) had lower average of services per conception and days open compared to cows with the +32:GG genotypes. However, no significant difference in the calving to first service was found between these genotypes. Further studies will be required to determine critical SNPs in LF gene and status of the risk of uterine infection and embryo survival in cows.
子宫感染是一种会影响奶牛产后子宫健康的生殖疾病。因此，识别对子宫感染具有抗性的基因型很重要。乳铁蛋白（LF）是奶牛正常子宫分泌物中的主要抗菌化合物之一。我们假设LF基因中的等位基因多样性可能导致对子宫感染的易感性或抗性。我们使用等位基因特异性PCR方法，对有子宫感染和无子宫感染的奶牛LF基因5'非翻译区（5'-UTR，位置+32）中鉴定出的单核苷酸多态性基因进行了研究。从89头有子宫感染病史的多胎荷斯坦奶牛（n = 51）和无疾病的奶牛作为对照组（n = 38）采集血样。结果表明，所有组奶牛的5'-UTR区域均存在不同比例的多态性（G > C）。等位基因特异性PCR结果与测序方法完全一致。统计分析未显示疾病与5'-UTR中的单核苷酸多态性之间存在任何统计学上的显著相关性。然而，与5'-UTR中有单核苷酸多态性的奶牛相比，无多态性的奶牛繁殖参数平均值存在显著差异。与+32:GG基因型的奶牛相比，+32:CC基因型和+32:GC基因型（UTR中有单核苷酸多态性的奶牛）的每受孕配种次数和空怀天数平均值较低。然而，这些基因型之间在产犊至首次配种方面未发现显著差异。需要进一步研究来确定LF基因中的关键单核苷酸多态性以及奶牛子宫感染风险和胚胎存活状况。
