Department of Animal Health and Poultry, Animal and Poultry Production Division, Desert Research Center (DRC), Cairo, Egypt.
Diagnostic Imaging and Endoscopy Unit, Agriculture Research Centre, Animal Reproduction Research Institute, Giza, Egypt.
Sci Rep. 2024 Mar 8;14(1):5698. doi: 10.1038/s41598-024-56258-0.
The objective of this study was to elaborate Doppler ultrasonographic scan, genetic resistance and serum profile of markers associated with endometritis susceptibility in Egyptian buffalo-cows. The enrolled animals were designed as; twenty five apparently healthy buffalo-cows considered as a control group and twenty five infected buffalo with endometritis. There were significant (p < 0.05) increased of cervical diameter, endometrium thickness, uterine horn diameter, TAMEAN, TAMAX and blood flow through middle uterine artery with significant decrease of PI and RI values in endometritis buffalo-cows. Gene expression levels were considerably higher in endometritis-affected buffaloes than in resistant ones for the genes A2M, ADAMTS20, KCNT2, MAP3K4, MAPK14, FKBP5, FCAMR, TLR2, IRAK3, CCl2, EPHA4, and iNOS. The RXFP1, NDUFS5, TGF-β, SOD3, CAT, and GPX genes were expressed at substantially lower levels in endometritis-affected buffaloes. The PCR-DNA sequence verdicts of healthy and affected buffaloes revealed differences in the SNPs in the amplified DNA bases related to endometritis for the investigated genes. However, MAP3K4 elicited a monomorphic pattern. There was a significant decrease of red blood cells (RBCs) count, Hb and packed cell volume (PCV) with neutrophilia, lymphocytosis and monocytosis in endometritis group compared with healthy ones. The serum levels of Hp, SAA, Cp, IL-6, IL-10, TNF-α, NO and MDA were significantly (P˂0.05) increased, along with reduction of CAT, GPx, SOD and TAC in buffalo-cows with endometritis compared to healthy ones. The variability of Doppler ultrasonographic scan and studied genes alongside alterations in the serum profile of investigated markers could be a reference guide for limiting buffalo endometritis through selective breeding of natural resistant animals.
本研究旨在详细阐述埃及水牛的多普勒超声扫描、遗传抗性和与子宫内膜炎易感性相关的血清标志物谱。纳入的动物设计为：二十五头表现健康的水牛作为对照组，二十五头感染子宫内膜炎的水牛。子宫内膜炎水牛的宫颈直径、子宫内膜厚度、子宫角直径、TAMEAN、TAMAX 和子宫中动脉血流显著增加（p<0.05），PI 和 RI 值显著降低。与抗性水牛相比，受子宫内膜炎影响的水牛中 A2M、ADAMTS20、KCNT2、MAP3K4、MAPK14、FKBP5、FCAMR、TLR2、IRAK3、CCl2、EPHA4 和 iNOS 基因的表达水平显著升高。与受子宫内膜炎影响的水牛相比，RXFP1、NDUFS5、TGF-β、SOD3、CAT 和 GPX 基因的表达水平显著降低。健康和受影响的水牛的 PCR-DNA 序列结果显示，与调查基因相关的扩增 DNA 碱基中的 SNPs 在受影响的水牛中存在差异。然而，MAP3K4 表现出单态性模式。与健康组相比，子宫内膜炎组的红细胞（RBC）计数、Hb 和红细胞压积（PCV）显著减少，中性粒细胞增多、淋巴细胞增多和单核细胞增多。与健康组相比，子宫内膜炎水牛的血清 Hp、SAA、Cp、IL-6、IL-10、TNF-α、NO 和 MDA 水平显著升高（P<0.05），CAT、GPx、SOD 和 TAC 水平降低。多普勒超声扫描和研究基因的变化以及研究标志物的血清谱的改变可以作为通过选择具有天然抗性的动物进行选择性繁殖来限制水牛子宫内膜炎的参考指南。
