用于模拟失重状态下基因表达研究的人骨髓单核细胞内参基因的鉴定
Identification of reference genes in human myelomonocytic cells for gene expression studies in altered gravity.
作者信息
Thiel Cora S, Hauschild Swantje, Tauber Svantje, Paulsen Katrin, Raig Christiane, Raem Arnold, Biskup Josefine, Gutewort Annett, Hürlimann Eva, Unverdorben Felix, Buttron Isabell, Lauber Beatrice, Philpot Claudia, Lier Hartwin, Engelmann Frank, Layer Liliana E, Ullrich Oliver
机构信息
Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraße 190, 8057 Zurich, Switzerland ; Department of Machine Design, Engineering Design and Product Development, Institute of Mechanical Engineering, Otto-von-Guericke-University Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany ; Study Group "Magdeburger Arbeitsgemeinschaft für Forschung unter Raumfahrt- und Schwerelosigkeitsbedingungen" (MARS), Otto-von-Guericke-University Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany.
Institute of Anatomy, Faculty of Medicine, University of Zurich, Winterthurerstraße 190, 8057 Zurich, Switzerland ; Department of Machine Design, Engineering Design and Product Development, Institute of Mechanical Engineering, Otto-von-Guericke-University Magdeburg, Universitätsplatz 2, 39106 Magdeburg, Germany.
出版信息
Biomed Res Int. 2015;2015:363575. doi: 10.1155/2015/363575. Epub 2015 Jan 13.
Gene expression studies are indispensable for investigation and elucidation of molecular mechanisms. For the process of normalization, reference genes ("housekeeping genes") are essential to verify gene expression analysis. Thus, it is assumed that these reference genes demonstrate similar expression levels over all experimental conditions. However, common recommendations about reference genes were established during 1 g conditions and therefore their applicability in studies with altered gravity has not been demonstrated yet. The microarray technology is frequently used to generate expression profiles under defined conditions and to determine the relative difference in expression levels between two or more different states. In our study, we searched for potential reference genes with stable expression during different gravitational conditions (microgravity, normogravity, and hypergravity) which are additionally not altered in different hardware systems. We were able to identify eight genes (ALB, B4GALT6, GAPDH, HMBS, YWHAZ, ABCA5, ABCA9, and ABCC1) which demonstrated no altered gene expression levels in all tested conditions and therefore represent good candidates for the standardization of gene expression studies in altered gravity.
基因表达研究对于分子机制的研究和阐明是不可或缺的。在标准化过程中，参考基因（“管家基因”）对于验证基因表达分析至关重要。因此，假定这些参考基因在所有实验条件下都表现出相似的表达水平。然而，关于参考基因的常见建议是在1g条件下确立的，因此它们在重力改变的研究中的适用性尚未得到证实。微阵列技术经常用于在特定条件下生成表达谱，并确定两个或多个不同状态之间表达水平的相对差异。在我们的研究中，我们寻找在不同重力条件（微重力、正常重力和超重力）下具有稳定表达且在不同硬件系统中也未发生改变的潜在参考基因。我们能够鉴定出八个基因（ALB、B4GALT6、GAPDH、HMBS、YWHAZ、ABCA5、ABCA9和ABCC1），它们在所有测试条件下基因表达水平均未改变，因此是重力改变情况下基因表达研究标准化的良好候选基因。
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